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<front>
<journal-meta>
<journal-id journal-id-type="pmc">IJAEB</journal-id>
<journal-id journal-id-type="nlm-ta">IJAEB</journal-id>
<journal-id journal-id-type="publisher-id">IJAEB</journal-id>
<journal-title-group>
<journal-title>International Journal of Agriculture, Environment and Biotechnology</journal-title>
</journal-title-group>
<issn pub-type="ppub">0974-1712</issn>
<issn pub-type="epub">2230-732X</issn>
<publisher>
<publisher-name>AAEB</publisher-name>
<publisher-loc>India</publisher-loc>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="other">IJAEB-14-1-75</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>AGRONOMY</subject>
</subj-group>
</article-categories>
<title-group>
<article-title>Evaluation of Maize (<italic>Zea mays</italic> L.) Varieties for Moisture Stress Areas in Humbo District, Wolaita Zone, Southern Ethiopia</article-title>
</title-group>
<contrib-group><contrib contrib-type="author">
<name><surname>Loha</surname><given-names>Gobeze</given-names></name><xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author">
<name><surname>Endale</surname><given-names>Abebe</given-names></name><xref ref-type="aff" rid="A2">2</xref>
</contrib>
<contrib contrib-type="author">
<name><surname>Tigabu</surname><given-names>Rahel</given-names></name><xref ref-type="aff" rid="A3">3</xref>
</contrib>
<contrib contrib-type="author">
<name><surname>Reshid</surname><given-names>Abdishikur</given-names></name><xref ref-type="aff" rid="A2">2</xref>
</contrib>
<contrib contrib-type="author">
<name><surname>Sorsa</surname><given-names>Zemach</given-names></name><xref ref-type="aff" rid="A4">4</xref>
</contrib>
<contrib contrib-type="author">
<name><surname>Senapathy</surname><given-names>M.</given-names>
</name>
<xref ref-type="aff" rid="A5">5</xref>
<xref ref-type="corresp" rid="cor001">*</xref></contrib></contrib-group>
<aff id="A1"><label>1</label>Department of Plant Sciences, College of Agriculture, Wolaita Sodo University, Ethiopia, East Africa</aff>
<aff id="A2"><label>2</label>Department of Plant Science, College of Agriculture, Wolaita Sodo University, Ethiopia, East Africa</aff>
<aff id="A3"><label>3</label>Department of Plant Science, College of Agriculture, Medawolabu University, Goba, Ethiopia, East Africa</aff>
<aff id="A4"><label>4</label>Department of Plant Science, College of Agriculture, Wolaita Sodo University, Ethiopia, East Africa</aff>
<aff id="A5"><label>5</label>Department of Rural Development and Agricultural Extension, College of Agriculture, Wolaita Sodo University, Ethiopia, East Africa</aff>
<author-notes><corresp id="cor001"><label>*</label>Corresponding author: <email>mspathy9@gmail.com</email> (<bold>ORCID ID:</bold> 0000-0002-8371-3035)</corresp>
</author-notes>
<pub-date pub-type="ppub">
<month>3</month>
<year iso-8601-date="2020">2021</year>
</pub-date>
<volume>14</volume>
<issue>1</issue>
<fpage>75</fpage>
<lpage>82</lpage>
<history>
<date date-type="received" iso-8601-date="2020-12-11">
<day>11</day>
<month>12</month>
<year>2020</year>
</date>
<date date-type="revised" iso-8601-date="2021-01-14">
<day>14</day>
<month>01</month>
<year>2021</year>
</date>
<date date-type="accepted" iso-8601-date="2021-02-28">
<day>28</day>
<month>02</month>
<year>2021</year>
</date>
</history>
<permissions>
<copyright-statement>&#x00A9; AAEB, India</copyright-statement>
<copyright-year>2020</copyright-year>
<copyright-holder>AAEB, India</copyright-holder>
</permissions>
<self-uri content-type="pdf" xlink:href="IJAEB-14-1-75.pdf"></self-uri>
<abstract>
<p>Maize is a priority crop to farmers because it is a staple food in many rural communities of the southern region. It is widely grown in the various parts of the southern region, from lowland to mid-highlands. On the other hand, moisture stress is one of the most critical production constraints of maize in low to intermediate agroecology. Thus, developing maize varieties tolerant to moisture is of paramount importance to sustain maize production in moisture areas. In this context, field experiments were conducted during 201617 croppings at Kindo Koyisha and Humbo to select adaptable maize varieties for moisture stress areas with reasonable grain yield. Treatments used in this study were eight maize varieties (BH546, BH547, Gibe II, MH130, Melkasa IV, MH140, Melkasa II, and Melkasa 6Q) and three local cultivars (Local red, Local mixed, and Local white) of the total of eleven maize genotypes were evaluated at two moisture-prone areas in southern Ethiopia. Treatments were laid out in a randomized complete block design (RCBD) with three replications. Maize varieties exhibited different performances at two tested locations. The varieties had the relatively superior performance of Kindo Koyisha as compared to Humbo. The Humbo varieties MH140, MH130, BH546, and Melkasa IV, have yielded relatively higher grain than the rest of the areas. At the same time, the Kindo Koyisha maize varieties expressed relatively better performance concerning grain yield. It has been recorded that the varieties with superior performance with sounding grain yield were BH546, MH140, BH547, and MH130 in these locations. Based on this result, BH546, MH140, and MH130 could be used at both locations. Moreover, BH547 at Bale and Melkasa IV at Humbo also be used in respective locations for production.</p>
<sec>
<title>Highlights</title>
<list list-type="bullet"><list-item><p>Complete set of genes inherited by an individual important for expressing a trait under consideration in a particular environment.</p></list-item>
<list-item><p>All maize varieties emerged as superior performer at Kindo Koyisha than that of Humbo.</p></list-item>
<list-item><p>BH546, MH140, and MH130 could be used at both locations for achieving potential sustainable yield.</p></list-item>
</list>
</sec>
</abstract>
<kwd-group>
<kwd>Maize varieties</kwd>
<kwd>moisture stress</kwd>
</kwd-group>
<counts>
<fig-count count="0"/>
<table-count count="5"/>
<ref-count count="17"/>
<page-count count="8"/>
</counts>
</article-meta>
</front>
<body>
<sec id="S1">
<title/>
<p><bold>How to cite this article:</bold> Loha, G., Endale, A., Tigabu, R., Reshid, A., Sorsa, Z. and Senapathy, M. 2021. Evaluation of Maize (<italic>Zea mays</italic> L.) Varieties for Moisture Stress Areas in Humbo District, Wolaita Zone, Southern Ethiopia. <italic>IJAEB,</italic><bold>14</bold>(1): 75-82.</p>
<p><bold>Source of Support:</bold> None; <bold>Conflict of Interest:</bold> None</p>
<p>Maize (<italic>Zea mays</italic> L.) has become the third most important cereal crop in the world because of its high adaptability and productivity (<xref ref-type="bibr" rid="R10">Mosisa <italic>et al.</italic> 2002</xref>). Globally, maize is cultivated under diverse climatic conditions but yields best under moderate temperatures with sufficient water (<xref ref-type="bibr" rid="R14">Parewa <italic>et al.</italic> 2010</xref>). The African continent is the essential food crop and mainstay of rural diets in the eastern and southern regions (FAO, 2003; <xref ref-type="bibr" rid="R9">Maredia <italic>et al.</italic> 2000</xref>; <xref ref-type="bibr" rid="R13">Pingali and Pandey, 2001</xref>). Maize has a higher carbohydrate production potential per unit of land than other cereals and was the first major cereal to undergo a rapid and widespread technological transformation in its cultivation (<xref ref-type="bibr" rid="R12">Palwal, 2000</xref>). In developed countries, maize is grown mainly for animal feed and raw materials for industrial products, such as starch, glucose, dextrose, and biofuel. Therefore, maize occupies an important position in Africa and the global economy, where it is traded as food, feed, and industrial grain crop (<xref ref-type="bibr" rid="R17">Vasal 2000</xref>).</p>
<p>In Ethiopia, cereals account for about 82.34% of the annual national crop production. Maize ranks first in total production and yield per unit area and second in area coverage among all the cereals. It is largely produced in western, central, southern, and eastern regions (CSA, 2010). Maize research has advanced from landraces to maize hybrids: double cross, three-way cross, single cross, and recently transgenic maize hybrids. The optimized use of adapted and exotic germplasm in various production environments is a key to the continued success in increasing grain yield and other trait-specific products: green ear, forage, oil, protein, starch.</p>
<p>Moreover, maize is a priority crop to farmers because it is a staple food in many rural communities of the southern region. It is widely grown in the various parts of the southern region, from lowland to mid-highlands. On the other hand, moisture stress is one of the most critical production constraints of maize in low to intermediate agroecology. However, the extent of yield reduction due to moisture stress varies with genotypes. Developing maize varieties tolerant to moisture is of paramount importance to sustain maize production in moisture areas. Hence, the objective of this study was to select adaptable maize varieties for moisture stress with reasonable grain yield.</p>
</sec>
<sec>
<title>MATERIALS AND METHODS</title>
<sec>
<title>Experimental site</title>
<p>Field experiments were conducted during 2016/17 cropping at Kindo Koyisha (Altitude 1170 masl, annual rainfall 924 mm, 2100, significant crops cultivated in the study area include maize, sorghum, and sweet potato) and Humbo (Altitude 1800, annual rainfall 1295 with bimodal rainfall patterns, average temperature of 20&#x00B0;C).</p>
</sec>
<sec>
<title>Treatments and experimental design</title>
<p>Treatments used in this study were eight maize varieties (BH546, BH547, Gibe II, MH130, Melkasa IV, MH140, Melkasa II, and Melkasa 6Q) and three local cultivars (Local red, Local mixed, and Local white) of the total of eleven maize genotypes were evaluated at two moisture-prone areas in southern Ethiopia. The Treatments were laid out in a Randomized Complete Block Design (RCBD) with three replications. The plot size was 4 &#x00D7; 4 m with 1.5 m between replications and 1.0 m between plots. Planting was carried out as per the planting time of the respective area following the onset of rainfall. Maize was hand planted by placing two seeds per hill and thinned after emergence to maintain the proposed plant density per plot. Weed control was carried out by hand or hand hoeing, while diseases and insect damage were visually monitored during crop growing season. Phosphorus fertilizer in the form of DAP and N in the form of urea was applied as per recommendation for maize production. Moreover, other crop management practices carried out as desired.</p>
</sec>
<sec>
<title>Data collection and measurements</title>
<p>Data recorded on yield components included ear length, ear diameter, number of seeds per row, kernels per ear, thousand seed weight, and prolificacy (ears per plant). Ear length and diameter were measured for five randomly selected plants from the base to the tip and approximately the middle of the ear at harvesting. The number of seeds per row was counted for five randomly selected plants. Seed number per ear was determined by multiplying the number of rows by the number of seeds per row. Thousand Seed Weight (TSW) was measured by counting a thousand seeds with a seed counter and weighing it with sensitive balance. Prolificacy is the property of producing more ears per plant and is estimated by dividing the number of ears by the number of plants per plot. Grain was manually harvested from the net plot and converted to kg/ha after adjusting the moisture content to 12.5%. Biomass yield was estimated as the sum of stover weighed and grain yield. Harvest Index (HI) is the ratio of grain yield to the total biomass yield, which was estimated by dividing grain yield by total biomass. Data were subjected to analysis of variance using the general linear model SAS version 9.1 (SAS Inst., 2003). Treatments means were compared using the least significant difference (LSD) at a 5% probability level.</p>
</sec>
</sec>
<sec>
<title>RESULTS AND DISCUSSION</title>
<sec>
<title>Plant and ear heights</title>
<p>The data for plant and ear heights as affected by location and varieties are depicted in <xref ref-type="table" rid="T1">Table 1</xref>.</p>
<table-wrap id="T1">
<label>Table 1</label>
<caption>
<p>Plant and ear heights as affected by location and varieties</p>
</caption>
<graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="IJAEB-14-1-75-t001.jpg"/>
</table-wrap>
<p>Analysis o variance indicated that location had a significant effect on plant and ear height. Both parameters were higher at Kindo Koyisha as compared to Humbo. Similarly, maize varieties have significantly differed for plant height and ear height. The tallest plant height (235 cm) was recorded for variety BH547, followed by variety BH547 with a mean plant height of 229 cm. The shortest plant height (156 cm) was seen for variety Melkasa 6Q. In line with this, the tallest ear heights (117 cm) were observed for BH547, followed by a variety of Local mixed with a mean ear height of 58 cm.</p>
<p>On the other hand, location by variety interactions resulted in significant differences in on-ear heights (<xref ref-type="table" rid="T1">Table 1</xref>). Generally, all varieties exhibited taller ear heights at Kindo Koyisha as compared to Humbo. The tallest ear height (148 cm) was observed for variety Local white at Humbo, followed by variety BH547 at Kindo Koyisha with a mean ear height of 147 cm. The shortest ear height (34 cm) was seen for variety Melkasa 6Q at Humbo. In contrast, location by varieties interaction did not have a significant effect on plant heights.</p>
</sec>
<sec>
<title>Ear length and ear diameter</title>
<p>The data for ear length and ear diameter as affected by location and varieties are presented in <xref ref-type="table" rid="T2">Table 2</xref>. Analysis of variance showed that the main effect of location and varieties had significant differences in length. The ear length of maize varieties was higher at Kindo Koyisha than that of Humbo. Averaged over locations, the longest cob length (15.13 cm) was obtained from variety BH546, followed by variety BH547 with a mean ear length of 13.74 cm. The shortest ear diameter (11.62 cm) was seen for variety Melkasa 6Q. In line with this, the interaction of location by varieties resulted in significant differences in ear length (<xref ref-type="table" rid="T2">Table 2</xref>). The most extended ear length (15.83 cm) was recorded for variety BH546 at Kindo Koyisha, followed by variety BH547 with a mean cob length of 14.45 cm at the exact location. The shortest ear length (9.69 cm) was seen for local white at Humbo.</p>
<p>On the other hand, the only main effect of varieties exhibited significant differences in ear length. The longest cob diameter (4.75 cm) was measured for variety BH546, followed by variety BH547 with a mean ear diameter of 4.34 cm. The shortest ear diameter (4.03 cm) was observed for local white. However, the main effect of location and its interactions with varieties did not significantly affect ear diameter.</p>
<table-wrap id="T2">
<label>Table 2</label>
<caption>
<p>Cob length and diameter as affected by location and varieties</p>
</caption>
<graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="IJAEB-14-1-75-t002.jpg"/>
</table-wrap>
</sec>
<sec>
<title>Rows per ear, seeds per row, seeds per ear, and thousand seed weight</title>
<p>The number of rows per ear seeds per row, seeds per ear, and TSW as affected by location and varieties are shown in <xref ref-type="table" rid="T3">Table 3</xref>.</p>
<table-wrap id="T3">
<label>Table 3</label>
<caption>
<p>Number of rows per cob, seeds per row, seeds per cob and TSW as affected by location and varieties</p>
</caption>
<graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="IJAEB-14-1-75-t003.jpg"/>
</table-wrap>
<p>Analysis of variance indicated that the main effect of location had a significant effect on the number of seeds per row and seeds per ear. Both parameters were higher at Kindo Koyisha as compared to that of Humbo. Similarly, varieties exhibited significant differences in rows per ear, seeds per row, seeds per ear, and TSW (<xref ref-type="table" rid="T3">Table 3</xref>). Variety BH547 produced the highest number per ear (15.1), followed by variety Melkasa 6Q with a mean number of rows per ear of 14.8. The least number of rows per cob (12.4) was seen for Local white. In line with this, the most significant number of seeds per row (34) and seeds per cob (469) were recorded for variety BH546, followed by variety BH547 with the mean number of seeds per row and seeds per ear of 30 and 445, respectively. Local white yielded the lowest number of seeds per row (26) and seeds per ear (295).</p>
<p>Moreover, location by variety interactions resulted in a significant number of seeds per row. The greatest number of seeds per row (36) was recorded that Kindo Koyisha for variety BH546, followed by the exact location for variety BH547 with the mean number of seeds per row of 34. The lowest number of seeds per row (22) was seen for Local white. In contrast, the main effect of location, variety, and interactions did not significantly affect TSW, seeds per row, and rows per ear (<xref ref-type="table" rid="T3">Table 3</xref>).</p>
</sec>
<sec>
<title>Biomass, grain yield and harvest index</title>
<p>The data for biomass, grain yield, and HI as affected by location and variety are depicted in <xref ref-type="table" rid="T4">Table 4</xref>. The location did not have a significant effect on the biomass yield of maize varieties. However, varieties exhibited significant differences in biomass yield. Biomass yield for maize varieties ranged from 7083 to 14792 kg/ha, with the highest biomass yield recorded (14792 kg/ha) for variety BH546, followed by variety BH547 biomass yield of 14688 kg/ha. The lowest biomass yield (7083 kg/ha) was obtained from variety Melkasa 6Q. In line with this, location by varieties interactions resulted in significant differences in biomass yield. The most excellent biomass yield (17188 kg/ha) was recorded at Kindo Koyisha for variety BH547, followed by variety BH546 at the exact location with a mean biomass yield of 15938 kg/ha. The lowest biomass yield (6979 kg/ha) was seen for variety Melkasa 6Q at Kindo Koyisha.</p>
<p>Grain yield has significantly differed in response to the location where higher grain yield was obtained from Kindo Koyisha than Humbo (<xref ref-type="table" rid="T3">Table 3</xref>). Similarly, maize varieties exhibited significant differences in grain yield. The highest grain yield (5208 kg/ha) was recorded at Kindo Koyisha for variety BH546, followed by MH140 with a mean grain yield of 5000 kg/ha at the exact location. The lowest grain yield (2396 kg/ha) was achieved from Local red at Humbo. In general, maize varieties tested for moisture responded differently to respective environments. At Kindo Koyisha, varieties BH546, MH140, and BH547 showed good performance in a such moisture-stress-prone environments. On the other hand, MH140 and MH130 relatively exhibited superiority over others at Humbo.</p>
<table-wrap id="T4">
<label>Table 4</label>
<caption>
<p>Biomass, grain yield and harvest index as affected by location and varieties</p>
</caption>
<graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="IJAEB-14-1-75-t004.jpg"/>
</table-wrap>
<p>Maize varieties exhibited differently for agronomic traits measured in response to the location regarding their genetic variability (<xref ref-type="table" rid="T1">Table 1</xref>, 2, 3, and 4). Generally, almost all maize varieties showed superior performance at Kindo Koyisha compared to Humbo for agronomic traits. The grain yield differences recorded were 730 kg/ha between Kindo Koyisha and Humbo. Thus, relatively the performances of varieties were poor at Humbo, which probably suggests that Kindo Koyisha was a relatively better environment with plant growth conditions. Moreover, this illustrated that subjecting plants to favourable growing conditions increased the ability of varieties to capture resources, which was reflected as evident in their increased agronomic performance. The significant effects of environments indicated that the genotypes performed differently across locations. Thus, the mean yield of genotypes differed from location to location.</p>
<p>Similarly, maize varieties, averaged over locations, showed significant differences in plant height, ear height, rows per cob, seeds per row, seeds per cob, ear length, and ear diameter (<xref ref-type="table" rid="T1">Table 1</xref>, 2 3). Relatively higher plant height (&#x2265; 200 cm) was recorded for varieties BH546, BH547, and MH140, whereas ear heights (&#x2265; 100 cm) were recorded for varieties BH547, Local mixed, and Local white. Variety BH546 gave the most extended cob length, while BH547 produced the highest cob diameter. Variety BH547 gave the highest number of rows per cob, while variety BH547 produced the most significant number of seeds per row and seeds per cob. Maize varieties averaged over locations tended to express a wide range of their genetic variability for grain yield. Grain yield variations ranged from 2396 to 4063 kg/ha. Variety BH 546 out yielded which MH140 followed. Local red was the least concerning grain yield performance. The significant difference among the genotypes showed variations in their response (yield potential) to different locations.</p>
<p>Location by variety interactions resulted in significant differences on-ear height, cob length, seeds per row, biomass, and grain yield (<xref ref-type="table" rid="T1">Table 1</xref>, 2 &#x0026; 3). For parameters as mentioned above, varieties had relatively superiority at Kindo Koyisha as compared to Humbo. In general, the performance of varieties was poor at Humbo, with the grain yield variability ranged from 2396 to 3021 kg/ha. At Humbo varieties, MH140, MH130, BH546, and Melkasa IV gave relatively higher grain yield with HI (Physiological efficiency and ability to convert total dry matter into economic yield) values 0.30, 0.40, 0.28, and 0.36, respectively. This variability might be attributed to varietal differences in maize genotypes in response to the prevailing environmental conditions. Hence, the Humbo location could be considered a stressful environment with profound limitations in the potential performance of maize varieties. At Kindo Koyisha, maize varieties expressed relatively better performance concerning grain yield. Grain yield variability ranged from 2604 to 5208 kg/ha from lowest to the highest. At this location, varieties with superior performance with sounding grain yield were BH546, MH140, BH547, and MH130. This probably indicates that genotypes describe the complete set of genes inherited by an individual important for expressing a trait under consideration in a particular environment.</p>
<p>In general, maize varieties at Kindo Koyisha performed best to their potential as compared to Humbo. Maize varieties BH546, MH130, and MH140 showed relative stability across the location with superiority of grain yield. The research results indicated that genotype by environment (G &#x00D7; E) interactions is a differential genotypic expression across environments which affects the genotypes rankings within each environment and hence relevant for identifying mega environments and targeting genotypes. Moreover, G &#x00D7; E&#x2019;s significance indicates fluctuation of genotypes performance across environments or testing sites with inconsistent performance. Similar results were recorded by <xref ref-type="bibr" rid="R4">Akcura <italic>et al.</italic> (2005)</xref>, Acura and Kaya (2008), <xref ref-type="bibr" rid="R5">Asfaw (2008)</xref>, <xref ref-type="bibr" rid="R7">Dagne (2008)</xref>, Solomon <italic>et al.</italic> (2008), <xref ref-type="bibr" rid="R1">Abdurhaman (2009)</xref>, and <xref ref-type="bibr" rid="R11">Muluken (2009)</xref>. The relationship between selected agronomic traits with the grain is depicted in <xref ref-type="table" rid="T5">Table 5</xref>. The correlation coefficient (r) values of selected agronomic traits with grain yield ranged from&#x2212;0.05 to 0.82. Plant and ear height were positively significantly (P &#x2264; 0.05) correlated, suggesting that the traits are closely associated with grain yield.</p>
<table-wrap id="T5">
<label>Table 5</label>
<caption>
<p>Correlation of growth and yield components with grain yield</p>
</caption>
<graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="IJAEB-14-1-75-t005.jpg"/>
</table-wrap>
<p>Similarly, the number of seeds per row, seeds per cob, ear length, ear diameter, biomass, and TSW were positively associated with yield. In contrast, the number of rows per cob with grain yield correlation was not significant. The correlation of almost all agronomic traits with grain yield was relatively strong, indicating that their contribution towards grain yield was considerable.</p>
</sec>
</sec>
<sec>
<title>CONCLUSION</title>
<p>Maize varieties reacted differently for agronomic traits measured in response to the location regarding their genetic variability. Generally, almost all maize varieties exhibited superior performance at Kindo Koyisha than that of Humbo. Based on this result, varieties BH546, MH140, and MH130 could be used at both locations. Moreover, varieties BH546 and MH 140 at Kindo Koyisha, whereas varieties MH 140, MH 130, and Melkasa IV at Humbo showed relatively better adaptation to their respective locations</p>
</sec>
</body>
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