International Journal of Agriculture, Environment & Biotechnology
Citation: IJAEB: 7 Special Issue : 409-419 July 2014
©2014 New Delhi Publishers. All rights reserved
Comparative analysis of Polyphenol Oxidase, Catalase and
Lycopene production in Lycopersicon esculentum Mill.
Ami Lokhandwala and Madhumati Bora*
Natubhai V Patel College of Pure and Applied Sciences, Vallabh Vidyanagar–388 120, Gujarat, India.
*Corresponding author : Madhumati Bora;
Antioxidant activity of tomato has been extensively studied but only in context of Lycopene. This study relates the activity of antioxidant
enzymes (Polyphenol Oxidase and Catalase) along with Lycopene in Lycopersicon esculentum Mill at different growth stages of (Seedling,
Flowering and Fruiting). Polyphenol Oxidase, Catalase (both partially purified) and Lycopene were estimated spectrophotometrically and
presence of Lycopene was further confirmed through Thin Layer and High-Performance Thin Layer Chromatography. Polyphenol Oxidase
and Catalase could be partially purified with 2.61 (22.55% yield) and 2.11 (62.3% yield) fold purification respectively via ammonium sulphate
precipitation respectively. Antioxidant enzymes showed maximum production at the seedling stage (Polyphenol Oxidases: 197.12U/ml and
Catalase: 0.037U/ml) where Lycopene production was least; while Lycopene production was maximum in the fruiting stage (259.49mg/kg
of fresh weigh) where enzyme activities were negligible. HPTLC analysis also supported the above findings. Linear Regression analysis
of Lycopene, PPO and CAT were performed in which r (correlation coefficient) value for Lycopene and PPO was -0.9279108012 and for
Lycopene and CAT was -0.7316992009; which indicated strong negative correlation between Lycopene and both the enzymes. It can be
concluded that Antioxidant enzymes play their share at young stages while Lycopene at mature stage in antioxidant network of tomato.
  • Open Pollinated varieties of Lycopersicon esculentum Mill have been used for analysis.
  • Enzymes have been partially purified via Ammonium Sulphate precipitation followed by dialysis and their physical parameters
    have been optimized.
  • Quantitative methods, Thin Layer chromatography and High Performance Thin Layer Chromatography have been used to study the
    production of Polyphenol Oxidases, Catalase and Lycopene.
    Keywords: Polyphenol Oxidase, Catalase, Lycopene, Lycopersicon esculentum Mill, Partial Purification and optimization.
    Abbreviations: Polyphenol Oxidases (PPOs), Catalase (CAT)
    anti-oxidative system comprising of non-enzymatic as
    stresses by altering their cellular metabolism and bringing
    well as enzymatic antioxidants in plants (Noctor and
    into play different defense mechanisms. Reactive species
    Foyer, 1998). Antioxidants can act by rummaging reactive
    are highly unstable structures; which are divided into
    oxygen species (e.g. Sulphur Oxide Dismutase removing
    four categories based on their central atom: Reactive
    oxygen), by inhibiting their formation (e.g. by blocking
    Oxygen Species (ROS), Reactive Nitrogen Species,
    phagocyte activation), by binding transition metal
    Reactive Chloride Species and Reactive Sulphur
    ions and preventing formation of hydroxyl ions and/
    Species (Halliwell and Gutteridge, 2007). Foraging or
    or decomposition of lipid hydro-peroxides, by repairing
    detoxification of excess ROS is attained by a competent
    damage (e.g. α –tocopherol repairing peroxyl radicals and