Lokhandwala and Bora

Table 1: Partial Purification of PPO and CAT from Lycopersicon esculentum Mill seedling

Volume

Protein

Total Protein Enzyme Specific Yield

Purification

Fraction

(ml)

(mg/ml)

(mg/ml)

(Units)

activity

(%)

fold

Crude Extract

100

4.5

450

511.8

1.13

100

1

PPO 50% Ammonium sulphate fraction

13

7.6

98.8

328.67

3.32

21.95

2.93

Dialyzed sample

14.5

7.0

101.5

300.18

2.95

22.55

2.61

Crude Extract

100

3.2

320

800.71

2,502

100

1

CAT 60% Ammonium sulphate fraction

13

6.9

89.7

506.92

5.651

63.308

2.2

Dialyzed sample

14.5

6.5

94.25

498.91

5.29

62.3

2.11

Table 2: Kinetic properties of partially purified PPO and CAT from Lycopersicon esculentum Mill seedling

Kinetic properties

PPO

CAT

Optimum pH range

7.0

7.0

Stable pH

8-10

7-9

Optimum temperature

40°C

70°C

Stable temperature range

10°C to 30°C

25°C to 40°C

Optimum Molarity

0.9 (sodium phosphate buffer pH 6.5)

0.1 (Potassium phosphate buffer pH 7.0)

Figure 1. Optimum pH profile for PPO and CAT

Figure 2. Optimum temperature for PPO and CAT

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