Comparative analysis of Polyphenol Oxidase
Kinetic studies for CAT
(ii)
Temperature:
The
optimum
temperature
(i)
pH: The optimum pH found by assaying enzyme
measured by assaying enzyme activity at various
activity at different pH levels was 7.0 in 0.05
temperatures was 70 o C (Figure 2, Table 2). The
M Potassium Phosphate buffer (Figure1, Table
activity obtained was 43.32 µmoles/ml/min.
2).The activity measured was 129.76 µmoles/ml/
CAT activity reduced to zero at 50 o C itself after
min.CAT activityreducedtozeroatpH1and2on
90 min of incubation; which indicates it is less
4 th and 5 th day of incubation respectively; which
stable at higher temperatures. The CAT enzyme
indicates that CAT was also stable at alkaline pH
was more stable between the range of 25 o C to
but PPO is more stable enzyme than CAT .CAT
40 o C (Figure 7a, 7b, 7c and Table 2).
was found more stable between the range of pH
7 to pH 9 in 0.05 M Potassium phosphate buffer
after incubating for 5 days (Figure 6a and 6b,
Table 2).
Figure 7a. Temperature stability profile for CAT (10°C,
25°C and 30°C)
Figure 7b. Temperature stability profile for CAT
Figure 6a and 6b. pH stability profile for CAT.
(40°C and 50°C)
415