Lokhandwala and Bora

Figure 8. Lycopene, PPO and CAT production at different

ontogenic stages in tomato plant.

Figure 7c. Temperature stability profile for CAT

(60°C, 70°C and 80°C)

(iii)

Ionic Strength: The optimum molarity for CAT

assayed was 0.1 M Potassium phosphate buffer

(pH7.0).Theactivityobtainedwas98.72µmoles/

min/ml (Figure 3, Table 2). CAT activity is

higher than PPO under their respective optimum

parameters though CAT is more unstable enzyme

than PPO at a particular parameter.

Lycopene, CAT and PPO at three ontogenic stages

Lycopene production was highest at fruit stage (259.49mg/

kg of fresh weight), while CAT and PPO activity was

Figure 9. HPTLC plate for Lycopene at different stages

under 285nm

highest at seedling stage (197.12U/ml and 0.037U/ml).

CAT and PPO activity was negligible at fruit stage, but

Lycopene production was observed at both seedling and

flowering stage; however, it was significantly less than

that produced at fruit stage which was confirmed by

HPTLC analysis also where Lycopene peak was highest

at fruit stage. Analysis also showed that PPO production

was extremely less as compared to CAT production at

both seedling and flowering stage (Figure 8, 9, 10 and

Table 3).

Statistical analysis

Correlation coefficient (r) obtained from Linear

Regression analysis between Lycopene, PPO and

CAT was -0.9279108012 for Lycopene and PPO while

Figure 10. Peaks obtained from HPTLC plate

(CAT software)

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