Genetic diversity and molecular analysis among cotton genotypes

Table 3. List of EST-SSR Primer their Quantitative Trait Loci (QTL) /Gene name and EST-SSR linked repeat sequence.

QTL/

EST-SSR Linked

Sl.No Primer sequence

Primer

QTL

Gene Name Repeat length

1.

TTTGGAGCCATTTACATGCA

BNL1030 (F)

QTL

qFL-D9-1

(GT)16 (CA)13

2.

AAACCACTTCTGCATCTGGA

BNL1030(R)

QTL

qFL-D9-1

(GT)16 (CA)13

3.

AAAAATCAGCCAAATTGGGA

BNL1317 (F)

QTL

qFL-D9-1

(AG)14

4.

CGTCAACAATTGTCCCAAGA

BNL1317(R)

QTL

qFL-D9-1

(AG)14

5.

AAAAACCCCTTTCCATCCAT

BNL1414(F)

QTL

qFL-D9-1

(AG)16

6.

GGGTGTCCTTCCCAAAAATT

BNL1414(R)

QTL

qFL-D9-1

(AG)16

7.

TAATAAAAGGGAAAGGAAAGAGTT

BNL1231(F)

QTL

qFS-A1-1

(AG)15

8.

TATGGCTCTAGAATATTCCCTCG

BNL1231(R)

QTL

qFS-A1-1

(AG)15

9.

CCTCCCTCACTTAAGGTGCA

BNL4030(F)

QTL

qFS-D-6-1

(GT)10

10.

ATGTTGTAAGGGTGCAAGGC

BNL4030(R)

QTL

qFS-D-6-1

(GT)10

11.

CACCATTGTGGCAACTGAGT

BNL3140(F)

QTL

qFS-D9-1

(GA)11

12.

GGAAAAGGGAAAGCCATTGT

BNL3140(R)

QTL

qFS-D9-1

(GA)11

13.

TCGATTTCCTTATTTGATTTCTG

BNL3650(F)

QTL

05 Lint

(TC)15+(TA)6

14.

AATTTGTCCAGATTCATTCTTCA

BNL3650(R)

QTL

05 Lint

TC)15+(TA)6

15.

ATCCAAACCATTGCACCACT

BNL3408(F)

QTL

LP

GT2AT(GT)12

16.

GTGTACGTTGAGAAGTCATCTGC

BNL3408(F)

QTL

LP

GT2AT(GT)12

17.

CATGTTCTAATCATATATATATGTATATATATGTGT

BNL3874(F)

QTL

LP

(AT)5+G+(TA)4(GT)10

18.

AAAATAACAAAAGCCATGGAATAA

BNL3874(R)

QTL

LP

(AT)5+G+(TA)4(GT)10

19.

TTGAGGGCATCCAAATCCAT

BNL3994(F)

QTL

LP

(CT)25+(GA)25

20.

CCTCCACCATACACGTGCTA

BNL3994(R)

QTL

LP

(CT)25+(GA)25

21.

ATTTGCCCCAGGTTTTTTTT

BNL3482(F)

QTL

LP

(AC)12

22.

GCAACACCTTTTCCTCCCTA

BNL3482(R)

QTL

LP

(AC)12

23.

TTCTTGCATTGAATAATACTGGC

BNL2920(F)

QTL

BW

(AG)12

24.

CTTAATTCTAAAAATCAATAA

BNL2920(R)

QTL

BW

(AG)12

25.

CTATGTTTGGCCTTGGCATT

BNL580(F)

QTL

BW

(GT)+(GT)+(CT)14

26.

TAGTGACAGATATCCCCGGC

BNL580(R)

QTL

BW

(GT)+(GT)+(CT)14

27.

ACCTGGGGTACTTGTCCACA

BNL341(F)

QTL

BW

(GA)14

28.

CCATCCCATTTGTGATACCC

BNL341(R)

QTL

BW

(GA)14

29.

TTAGGGTTTAGTTGAATGG

CIR246(F)

QTL

qFL-D2-1

(TG)6

30.

ATGAACACACGCACG

CIR246(R)

QTL

qFL-D2-1

(TG)6

31.

ACTAGCAGTGCGAATACA

CIR45(F)

QTL

qFL-D2-1

(TG)7

32.

TGGTTAAGGGTTGGG

CIR45(R)

QTL

qFL-D2-1

(TG)7

33.

CAATATCTCACTTGGACCT

CIR30(F)

QTL

LP

(C)8(TC)6(CA)8

34.

TGCTACACATCATAGTTGG

CIR30(R)

QTL

LP

(C)8(TC)6(CA)8

35.

TGCATGATGAAGTTAGA

CIR78(F)

QTL

LP

(GT)7

36.

ACATAAATCCCAAGAAC

CIR78(R)

QTL

LP

(GT)7

37.

CTTCATCATAGTAGCGAGTT

CIR182(F)

QTL

LP

(AC)10

443