Mishra and Fougat
in all clusters included Gvhv 235. Second cluster formed
they are also abundant in both diploid ( G. herbaceum,
two subgroups viz. A1 and A2. Cluster A1 included two
G.arboreum ) genotypes therefore, they are best markers
genotypes Digvijay and Dhumad. The A2 cluster again
for marker assisted selection (MAS) in both diploid ( G.
formed two sub group viz. A2A and A2B. A2A includes
herbaceum and G.arboreum ) genotypes for fibre quality
11 genotypes viz. Gvhv133, G.cot16, G.cotH10, G.cot
traits. Fibre quality linked SSR markers of tetraploid
20, G.cot8, CINA329, CINA318, CINA344, Jawar Tapti,
cotton in diploid species may be used to amplify DNA
CINA333 and DLSA17. A2B includes 10 genotypes viz.
fragments in most of diploids genome.
G.cotH4, G.cot12, 824, G. cot21, Gvhv104, Gvhv505,
EST-SSR markers used for this study reveals diversity
G.cot13, G.cot13, V-797 and Gvhv544. Gvhv133, G.cot
and similarities among and between genotypes of diploid
16, G.cotH10, G.cot20, G.cot8, CINA329, CINA318,
and tetraploid cottons genotypes based on molecular
G27, Jawar Tapti, CINA333 and DLSA17 which leads
characterization through EST-SSR Primers.
us to assume that the A2A subgroup cluster included the
varieties of similar genetic origin and which can be helpful
Conclusion
for consideration of these varieties for the hybridization
programme, while the A2B subgroup cluster contained
Comparative study between diploid and tetraploid cotton
G.cotH4, G.cot12, 824, G. cot21, Gvhv104, Gvhv505,
species for various fibre quality traits indicated that
G.cot13, G.cot13, V-797 and Gvhv544. The involvement
tetraploid-derived EST SSR primers for fibre quality
of genotypes in the same cluster again indicated that
traits could be successfully used in diploid species of
the varieties with the same genetic composition were
cotton for marker assisted breeding. Large portions
falling in the one cluster and this can help to conduct
of EST-SSR flanking regions appear to be conserved
a hybridization programme with these genotypes. The
among diploid and tetraploid genomes of cotton. EST-
similarity coefficients ranged from 0.05 to 0.71 for all
SSR markers amplified by primer CIR 30, NAU1200,
accessions. Thus, the relatedness of the cultivars studied
NAU4024, NAU1531, BNL2920, JESPR208, JESPR127
was efficiently established through the use of SSR markers
and NAU923 gave high polymorphism and they are also
though with some differences in the positioning of some
abundant in both diploid ( G. herbaceum,G.arboreum )
cultivars at various clusters. Both the dendrograms reflect
species therefore, they are best markers for marker
relationships among most of the cultivars, depending
assisted selection (MAS) in both diploid ( G. herbaceum
upon their fiber quality traits.
and G.arboreum ) species for fibre quality traits. These
studies also determined that tetraploid derived EST SSR
Application of Tetraploid Derived EST SSR Primers in
marker primers could be used to amplify DNA fragment
Diploid cotton Species.
in diploid cotton species.
The electrophoretic profiles obtained with the study
Dendrograms generated for EST- SSR markers of cotton
of the primers, 31 primers gave amplification in both
genotypes reflect relationships among most of the
diploids and tetraploids, out of which 26 primers gave
diploids ( G. herbaceum, G.arboreum ) and tetraploids
polymorphism in both diploids and tetraploids. JESPR29,
( G.hirsutum ) cultivars, depending upon their fibre quality
NAU3260,
BNL3874,
CIR70,
NAU3654,
CIR78,
traits. The involvement of genotypes in the same cluster
BNL1030, BNL3482, NAU3393, BNL3650, CIR45,
again indicated that the varieties with the same genetic
BNL1414, CIR307, BNL341, BNL580, BNL3408,
composition were falling in the one cluster and this can
BNL1317, BNL4030, CIR30, NAU1200, NAU4024,
help to conduct a hybridization programme with these
NAU1531,
BNL2920,
JESPR208,
JESPR127
and
genotypes.
NAU923 produced significant polymorphism among the
G. herbaceum, G.arboreum and G.hirsutum, genotypes
References
analyzed. EST-SSR markers amplified by primer CIR 30,
Abdurakhmonov IY, Buriev ZT, Saha S, Pepper AE, et al. 2007.
NAU1200, NAU4024, NAU1531, BNL2920, JESPR208,
Microsatellite markers associated with lint percentage
JESPR127 and NAU923 gave high polymorphism and
trait in cotton, Gossypium hirsutum. Euphytica 156: 141-
156.
448