International Journal of Agriculture, Environment & Biotechnology
Citation: IJAEB: 7 Special Issue : 467-478 July 2014
©2014 New Delhi Publishers. All rights reserved
Chronic toxic effect of Acenapthene on diverse microalgaes
and cyanobacteria: Chlorella vulgaris Beijerinck,
Desmodesmus subspicatus Chodat and Scytonema sp.
Miral S. Patel*¹, J.I. Nirmal Kumar² and K.K.Tiwari¹
1 Sophisticated Instrumentation Center for Applied Research and Testing (SICART), Vallabh Vidya Nagar, Gujarat, India- 388 120.
2 P.G. Department of environmental Science and Technology, Institute of Science and Technology for
Advanced Studies and Research (ISTAR), Vallabh Vidya Nagar, Gujarat, India- 388 120.
*Corresponding author: Miral Patel;
There is an increasing need to describe the growth characteristics of algae exposed to polycyclic aromatic hydrocarbons (PAHs) because of
occurrence of PAHs in lakes is known to deleteriously affect the growth of microorganisms. This study explored the chronic effect of different
doses of three ring structure polycyclic hydrocarbon Acenapthene on the growth of two microalgal species and one cyanobacterial species.
C. vulgaris , D. subspicatus and Scytonema sp. cultivated in the medium with different concentration of PAH and its affect was investigated
during increasing 4, 8, 12 and 16 Days of exposure. The growth of Chlorella vulgaris , Desmodesmus subspicatus and Scytonema sp . was
adversely affected by Acenapthene. The results indicated that the increased concentration of Acenapthene negatively impacted on chlorophyll
content, carotenoids, phycobilliproteins, carbohydrate, amino acids, proteins, nitrate reductase, succinate dehydrogenase and glutamate
synthetase except phenol . However, the raise in Phenol content was observed during the incubation period. Moreover, a high significance
correlation (F>0.05) existed between different metabolites, pigments and enzymes which was statistically confirmed by Two Way Analysis
of variance (ANOVA).
  • In the present study an attempt has been made to determine the chronic effects of various concentrations of PAH acenapthene on
    pigments like total chlorophyll, carotenoids, phycocyanin, allophycocyanin, and phycoerythrene, as well as on the biochemical
    compounds, C/N ratio and nitrate reductase, succinate dehydrogenase and glutamate synthetase of the two important microalgal
    species C. vulgaris and D. subspicatus and a cynobacterialspeciesScytonema.
  • It was proved that acenapthene found more toxicity to D. subspicatus>C. vulgarsi>Scytonema sp. and revealed thatincreased
    concentrations of acenapthene doses subsequently decline different metabolic, enzymatic activities and pigments of species of
    microalgae and cynobaceteria.
    Keywords: Acenapthene, microalgae, photosynthetic pigments, biochemical compounds, C/N ratio, enzymatic activities.
    Aquatic ecosystems are affected by a number of toxic
    to microorganisms as well as to higher living systems
    compounds including polycyclic aromatic hydrocarbons
    including humans (Samanta et al., 2002). Contamination
    (PAHs). These lipophilic organic pollutants represent
    of polycyclic aromatic hydrocarbons (PAHs) in the
    a group of several hundred individual compounds
    environment has attracted increasing public and academic
    containing at least two condensed rings (Wilcke et al. ,
    concern because some of them are known or suspected
    2002). Many PAHs and their derivatives, especially
    mutagens or carcinogens. PAHs are produced into the
    epoxides, are highly toxic, mutagenic and/or carcinogenic
    environment through natural and anthropogenic sources.