Patel et al.

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Adhikary, S. P., P. Das, and H. Pattnaik. 1984. Effect of carbamate

prolifica and quoted similar results. Glutamine synthetase

insecticide Sevin on Anabaena sp. and Westiellopsis

(GS) leads to the conversion of ammonia to glutamine

prolifica. Journal of General Applied Microbiology 35 :

335-338.

and also expressed a concentration dependent inhibition

when treated with the PAH. The GS activities of C.

Atlas, R.M., E. A., Schofield, F. A. Moreli, and R. E. Cameron.

1976. Effects of petroleum pollutants on Arctic microbial

vulgaris , D. subspicatus and Scytonema sp. suppressed

populations. Environmental Pollution 10 :35-45.

by 92%, 90% and 91% (fig. 3b) after Acenapthene

Baker, J.M. 1971. Comparative toxicities of oils, oil fractions

treatments respectively which has also been further

and emulsifiers. In E. B. Colwell (ed.), p. 78-87. The

supported by Rajendran et al. (2007) expressing a

ecological effect of oil pollution on littoral communities.

remarkable decrease in the GS activity on utilization of

Institute of Petroleum, London.

pesticides. Succinate dehydrogenase (SDH) enzyme is

Bennett, A. and L. Bogorad. 1973. Complementary chromatic

a major respiratory enzyme responsible for conversion

adaptation in a filamentous blue-green alga. Journal of

of succinate to fumarate in the tricarboxylic acid cycle

Cell Biol o gy 58 :419-435.

(TCA). But the treatment of this PAH (Acenapthene)

Burkhardt, S., I. Zondervan, U. Riebesell. 1999. Effects of CO ₂

lowered the activities of C. vulgaris , D. subspicatus

concentration on C:N:P ratio in marine phytoplankton: a

and Scytonema sp. by 89%, 87% and 88% respectively

species comparison, Limnol. Oceanogr. 44(3) :683–690.

after 16 days (fig. 3c). Similar inhibition of the enzyme

Canet, .R, J. G. Birnstingl, D. G. Malcolm, J. M. Lopez-Real,

succinate dehydrogenase activity was observed in the

and A. J. Beck. 2001. Biodegradation of polycyclic

aromatic hydrocarbons (PAHs) by native microXora and

cultures of four Gram (+) bacteria, Rhodococcus Sp. AK 1 ,

combinations of white-rot fungi in a coal-tar contaminated

Bacillus cereus Frankland & Frankland, Bacillus subtilis

soil. Bioresource Technol o gy 76 :113–117.

(Ehrenberg) Cohn, Nocardia asteroides and a Gram (-)

Carman, K.R., J. W. Fleeger, and S. M. Pomarico. 1997.

bacterium, Rhizobium leguminosarum when treated with

Response of benthic food web to hydrocarbon

the fungicide tridenmorph by Kalam et al., (1995).

contamination, Limnol. Oceanogr . 42 (3) :561–571.

Chabbi, A., C. Rumpel. 2004. Chemical composition of

Statistical Analysis

organic matter in extremely acid, lignite-containing lake

ANOVA: All the parameters found to be non- significant

sediments impacted by fly ash contamination. Journal of

Environmental Qual. 33 (2) :628–636.

(P>0.05) between the pigments, metabolites, enzymatic

Coffey, J.C., C. H. Ward, and J. M. King, 1977. Effect of

activities and concentrations applied (Table 2).

petroleum hydrocarbons on growth of freshwater algae.

Dev. Ind. Microbiol 18 :661-672.

Conclusion

Gordon, D.C. and N. J. Prouse. 1973. The effects of three oils

In the present investigation the microalgal cells were

on marine phytoplankton photosynthesis. Mar. Biol .

exposed to constant concentrations of PAHs- Acenapthene

22 :329-333.

which proved more toxicity to D. subspicatus>C.

Gunkel, W. and G. Gassmann. 1980. Hydrocarbons and

vulgarsi>Scytonema sp . It has been revealed that increased

hydrocarbon degradation in the marine environment

concentrations of Acenapthene doses subsequently

including some considerations of the water-sediment

interface. Colloq. Int. Cent. Natl. Rech. Sci. 293 :301-307.

decline different metabolites, enzyme activities and other

pigments of microalgae and cynobaceteria studied.

Huang, L., D. Lu, J. Diao, and Z. Zhou. 2012. Enantioselective

toxic effects and biodegradation of benalaxyl in

Scenedesmus obliquus. Chemosphere 87 :7-11.

Acknowledgement

Jeffrey,S.W.andG.F.Humphrey.1975.Newspectrophotometric

The authors would like to thank SICART for its support

equations for determining chlorophylls a, b, c1 and c2 in

in laboratory as well as instrumentation facilities.

higher plants, algae and natural populations. Biochem.

Physiol. Pflanzen. 167 : 191-194.

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