Patel et al.
Analysis of EPS as emulsifier
rpm for 4 days. The flasks containing basal medium
In clean glass test tubes, 10 ml of different oils was
were supplemented with variable aluminum and iron
added. The tubes were inverted and oil were removed
concentration (200, 400, 600 mg/ml). The samples were
in such a way that the portion of oil should stick to the
withdrawn and centrifuged at 6000 rpm for 10 min to
walls of the tube. 1 ml of EPS sample added drop by
separate cell biomass and supernatants were used to
drop, very slowly in each tube by holding the tube in
determine metal removal at different time interval. The
horizontal position. The inner surface of the test tube
supernatant obtained was used to determine aluminum
observed carefully for dispersion of the oil layer (Jagtap
and iron estimation as mentioned earlier.
et al., 2010).
3. Results and Discussion
Various metal removal efficiency determinations using
Isolation and Screening of EPS producing fungal
fungal isolates
cultures
To investigate the role of EPS in metal removal, the basal
Different fungal cultures were screened out for their EPS
medium was supplemented with various metals salts
producing ability. Six fungal isolates (SGMP1, SGMP2,
(K 2 Cr 2 O 7 , FeCl 3 , and AlCl 3 ) at the concentration of 100
SGMP3, SGMP4, SGMP5 and SGMP6) were obtained
mg/ml. The flasks were inoculated with actively grown
among them maximum production was obtained using
fungal isolate and incubated at 28°C on shaker at 120 rpm
fungal culture SGMP1 and SGMP2.
for 24-72 hours. The samples were withdrawn at different
time intervals. The samples were centrifuged at 6000
Effect of Different Carbon Sources on EPS Production
rpm for 10 min to separate cell biomass and supernatants
were used to determine metal removal efficiency. For
Carbohydrates are important nutritional requirement
estimation of different metals standard methods were
for the growth and development. To determine the role
used as described in APHA, (1995).
of suitable carbon source on EPS production the basal
medium supplemented with different carbon sources,
Effect of initial aluminum and iron concentration on
among various carbon sources, maximum EPS production
metal removal using fungal isolates
The flasks were inoculated with actively grown
fungal isolate and incubated at 28 0 C on shaker at 120
Fig 1(a): Effect of Carbon source on EPS
Fig 1(b): Effect of Carbon source on EPS production
production by SGMP1 fungal isolate
by SGMP2 fungal isolate
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