Production of Exopolysaccharide

(20.5 g/l) and dry cell biomass (3.1 g/l) were obtained

concentration in nutritional medium showed, 2% of

in case of by SGMP1 with glucose as a suitable carbon

glucose is sufficient to ensure the maximal biomass

source as shown in Fig. 1(a). However, SGMP2 isolate

yield (20.5 g/l) for SGMP1 (Fig. 2(a)). Further increase

showed maximum EPS production and dry cell biomass

in glucose/starch concentration leads to decreased EPS

(7.5 g/l, 6.5g/l) were observed with supplementation

production due to feedback inhibition. Jong et al., (2004)

of starch (Fig. 1(b)). This indicates supplementation of

reported that using Sarcodon aspartus fungal strain

simple carbon source in one fungal isolate enhances the

showed a maximum EPS production with 2% glucose

EPS production while other fugal isolate showed high

concentration however further rise in glucose showed

EPS production with supplementation of complex sugar

decreased EPS production. Whereas as shown Fig.

source. Similarly, Nehad and El-Shamy (2010) reported

2(b) shows the fungal isolate SGMP2 gave maximum

maximum production (16.3 mg/ml) of EPS using glucose

EPS production 7.9 g/l by using 2% starch as a carbon

as a carbon source. In contrast significant production of

source. Further increase in starch concentration leads to

EPS (1.87 g/l) was reported with supplementation of

decreased EPS production, because a high concentration

fructose in medicinal fungus Trametes versicolor (Bolla

of starch increased medium viscosity. The results obtained

et al., 2010).

in case of fungi SGMP2 are in accordance to the results

Furthermore, to determine an effective concentration

reported by Hamedi et al., (2007) who described that

of carbon source in the flasks were supplemented

Agaricus blazei utilized 2% starch and gave maximum

with variable concentrations. The variation of glucose

EPS production.

Fig 2(a): Effect of Initial glucose concentrations

Fig 2(b). Effect of Initial starch concentrations

on EPS production by SGMP1 fungal isolate

on EPS production by SGMP2 fungal isolate

Fig 3(a): Effect of nitrogen source on EPS

Fig 3(b). Effect of nitrogen source on EPS

production by SGMP1 fungal isolate

production by SGMP2 fungal isolate

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