Patel et al.
Effect of Different Nitrogen Sources on EPS Production
investigate the effect of pH on the EPS production, the
Nitrogen is one of the key nutrients for fungus in the
initial pH of the medium was adjusted in the range of
synthesis of enzymes involved in the production of both
3.5 to 6. The result showed at pH 5.5 maximum EPS
primary and secondary metabolites. To investigate the
production and cell biomass of SGMP1 (25 g/l and 21.9
effect of nitrogen source, both organic and inorganic
g/l) and SGMP2 (7.5g/l and 6.5 g/l) were observed (Fig 5
nitrogen sources were examined. Amongst various
(a) and 5 (b)). It indicates that acidic condition favors the
nitrogen sources used, yeast extract (organic nitrogen
EPS production. At high pH value, the EPS production
source) was found to be the most significant in influencing
declines sharply as alkaline pH is not suitable for fungal
mycelial growth and EPS production, while the presence
growth. Nguyen et al., (2012) reported 5.29 mg/ml EPS
of inorganic nitrogen sources were less effective and
produced at pH 5.5 in medicinal mushroom Grifola
showed poor biomass and EPS production (Banerjee et
frondosa . The high yield of EPS and mycelial growth
al., 2009). As shown in Fig. 3 (a) and 3(b), the maximum
(10.56 g/l and 0.854 g/l) were also reported between
EPS production 21.5 g/l and 7.5 g/l were observed for
pH 5 and 6 in Hericium erinaceus (Kim et al., 2002).
fungal isolates SGMP1 and SGMP2, respectively. The
The time course study of EPS production
results obtained are in agreement with those of Huang
et al., (2007) who studied the addition of yeast extract in
The incubation period is very crucial factor for the EPS
the basal medium showed maximum EPS production 4
production. The time course study of EPS production
g/l in case of Hericium erinaceus .
shows that with an increase in incubation period there is
Fig 4(a). Effect of Yeast Extract concentrations on
Fig 4(b). Effect of Yeast Extract concentrations on EPS
EPS production by SGMP1 fungal isolate
production by SGMP2 fungal isolate
Furthermore, to optimize the yeast extract concentration,
an increase in EPS production. However, EPS production
medium contained 0.1% of yeast extract showed maximum
on 4 th , 5 th and 6 th day almost remained constant. The
EPS production as shown in Fig. 4(a) and 4(b). Nehad
EPS production of SGMP1 and SGMP2 were 20.5 g/l
and El-Shamy (2010) had also described significant EPS
and 7.5 g/l, respectively on 4 th day (Fig. 6(a) and 6(b)).
production (4.5 g/l) at the concentration of 2 % yeast
Rong L. et al, (2010) suggested that Hirsutella sp. gives
concentration using fungal culture A. alternate.
highest EPS production and mycelial biomass (7.94 g/l,
1.12 g/l) on the 4 th day of incubation period. The highest
Effect of pH on EPS Production
EPS production 11.69 mg/ml was observed in Alternania
The pH of the culture medium is a vital factor that
alternate on 9 th day by Nehad and El -Shamy (2010).
governs mycelial growth and EPS production. To
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