Production of Exopolysaccharide

Fig 5 (a): Effect of pH on EPS production by

Fig 5 (b): Effect of pH on EPS production

SGMP1 fungal isolate

by SGMP2 fungal isolate

Fig 6 (a): Effect of incubation period on

Fig 6 (b): Effect of incubation period

EPS production by SGMP1 fungal isolate

on EPS production by SGMP2 fungal isolate

Fourier

Transform

Infrared

Spectrophotometer

Antimicrobial activity of EPS

(FTIR)

EPS samples of both the fungal isolates were investigated

IR spectrum of crude EPS obtained from SGMP1 and

for antimicrobial activity against different pathogenic

SGMP2 revealed the characteristic functional groups of

gram positive and gram negative bacterial strains. As

EPS (Fig. 7(a) and 7(b)). The sharp peak in the range of

shown in Fig. 8, the zone of inhibition against gram-

1400-1500 cm -1 indicates the presence of polysaccharide,

positive Staphylococcus aureus and gram-negative

carboxylic acids and lactones which suffices the nature

Salmonella typhi, Pseudomonas aeruginosa , Escherichia

of polysaccharide. In the spectra of SGMP1 EPS, the

coli were observed. Saskiawan (2009) also reported the

sharp peak at 1200-1000 cm -1 also strongly suggests the

antimicrobial activity of EPS produced by P. ostreatus

presence of phosphorus compounds. In the spectra of

against E.coli, B. subtilis and S. cerevisiae .

SGMP2 EPS, the sharp peak at 1631.45 cm -1 strongly

suggest presence of vinyl alkenes with C=O stretching.

517