Patel et al.

polysaccharides can act as electron donor to react with

Determination of flocculating activity

free radicals to from stable products (Vamanu et al.,

Qin et al., (2007) suggested that EPS could make

2012).

colloidal and suspended particle in solution which

2. Hydrogen peroxide scavenging assay

justify the EPS is a good flocculating agent and has a

good adsorptive capacity. Particles of charcoal were

The hydrogen peroxide inhibition activity of EPS and

effectively aggregated and precipitated by the addition of

ascorbic acid was increased with increase in incubation

the EPS. With charcoal as the suspended particles, the

time. The scavenging activity of EPS obtained from fungal

FA of the crude supernatant reached, approximately, 99%

isolate SGMP2 was higher than that of the ascorbic acid

flocculating activity without addition of cations such as

and the scavenging activity of EPS obtained from fungal

Ca 2+ and Mg 2+. However, addition of Ca 2+ or Mg 2+ was

isolate SGMP1 was lower than that of the ascorbic acid

found to be necessary to achieve maximum FA of fungal

(Fig.10 (b)). Hydrogen peroxide scavenging activity of

EPS produced by Mucor sp . (Abdel-Aziz et al., 2012).

EPS obtained from marine filamentous fungi Keissleriella

sp. YS 4108 was concentration dependent and maximum

Table 1. EPS production of different isolates

activity 72 % was observed at a concentration of 133 μg/

Culture

EPS (g/l)

Dry cell Biomass (g/l)

ml (Li et al., 2012).

SGMP1

17

7.3

3. ABTS Inhibition assay

SGMP2

15.25

4.1

As shown in Fig.10 (b), the ABTS inhibition activity

SGMP3

4.25

5.4

of EPS and ascorbic acid was increased with increase

SGMP4

3.0

1.0

in concentration of EPS and ascorbic acid. The results

indicate that EPS of fungal origin possessed scavenging

Table 2. Antimicrobial activities of SGMP1 and SGMP2

power for ABTS radicals. Similar type of scavenging

fungal culture against test organism

power was reported in obtained EPS from edible

Test organism

SGMP1(mm)

SGMP2(mm)

mushroom P. ostreatus (Vamanu et al., 2012) and in

Staphylococcus aureus

3

5

thermophilic green alga Cosmarium sp. (Challouf et al.,

Salmonella typhi

9

5

2012).

Escherichia coli

8

7

Pseudomonas aeruginosa

6

8

Fig 11(a). various Metal Removal efficiency using fungal

Fig 11(b): Effect of initial Fe +3 on Fe removal efficiency using

isolates SGMP1 and SGMP2

fungal isolates SGMP1 and SGMP2

520