International Journal of Agriculture, Environment & Biotechnology
Citation: IJAEB: 7 Special Issue : 499-510 July 2014
©2014 New Delhi Publishers. All rights reserved
Biological removal of synthetic textile dye Reactive Red
M5B by isolated white rot fungal culture AGYP-1 under
optimized culture conditions
Yogesh Patel and Akshaya Gupte*
Natubhai V. Patel College of Pure and Applied Sciences, Vallabh Vidyanagar, Anand 388 120, Gujarat, India.
*Corresponding author: Akshaya Gupte; firstname.lastname@example.org
The contamination of soil and water by dye containing effluents is the major and most important environmental problem. The removal of
10 different synthetic textile dyes using white rot fungal isolate AGYP-1 was investigated. The screening of decolorization using solid and
liquid media demonstrated an effective removal of Reactive Red M5B by the isolate. Laccase and MnP were found to be major enzymes
involved in the decolorization. The dye decolorization efficiency of the isolate was further improved by optimizing various physico-chemical
parameters. The isolate was capable to decolorize 100 mg l -1 dye at pH 5.0 and 30 o C under shaking condition. The supplementation of maltose
(20 g l -1 ) and gelatine (2.5 g l -1 ) improved the decolorization rate by 1.6 times along with 10.28 and 18.66 times higher production of laccase
and MnP. A significant decolorization of 500 mg l -1 of the dye was achieved by the isolate AGYP-1. The degradation of Reactive Red M5B
was confirmed by Uv-visible spectrophotometric and HPTLC analysis. This suggests the potential application of the isolate AGYP-1 for the
treatment of dye containing industrial effluents.
Keywords: Decolorization, Reactive Red M5B, Laccase, MnP, optimization
Isolation and Screening of different white rot fungi was performed using solid and liquid culture media. An isolate AGYP-1
decolorized 10 different textile dyes in liquid medium.
The effect of various physico-chemical parameters was studied for the decolorization of model dye Reactive Red M5B by isolate
AGYP-1 using single factorial design.
The isolate AGYP-1 was able to decolorize 500 mg l-1 Reactive Red M5B with an apparent maximum decolorization rate (RDmax)
and Michaelis constant (Km) 128.20 mg dye day-1 and 166.66 mg l-1 respectively.
UV-Visible spectroscopy and HPTLC analysis revealed the formation of new metabolites or metabolic intermediates different from
the parent dye Reactive Red M5B.
The global population expansion and an industrial based
an enormous amount of dye containing wastewaters
life style have created an anthropogenic impact on the
(Balaji et al ., 2012). Azo dyes with one or more (–N=N-)
biosphere. A variety of toxic compounds released from
and sulfonic (-SO 3 - ) groups are the most important
different industries adversely affect water source, soil
and widely used synthetic dyes having broad range of
fertility, aquatic organisms and ecosystem integrity
applications (Patel et al ., 2012). The discharge of poorly
(Khalid et al ., 2008). The textile and paper industries are
treated dye containing effluent alters the pH, increases
considered as the largest water consumers and discharge
the biochemical oxygen demand (BOD) and chemical