Prajapati et al.

oxygen demand (COD) of the aquatic system as well as

degrade a wide range of recalcitrant organic compounds.

reduces sunlight penetration leading to negative impact

They produce an array of lignin modifying enzymes

on the water quality (Carias et al ., 2007). Many dyes

(LME) such as Manganese peroxidase (MnP), lignin

and their transformation products are toxic, mutagenic

peroxidase (LiP) and laccases, which degrade not only

and carcinogenic that can endanger human life (Bae and

lignin in their natural lignocellulosic substrates, but also

Freeman, 2007). Therefore, it is a great need to develop

various xenobiotic compounds including dyes (Kalpana

an economic and effective way for the treatment of textile

et al ., 2012; Asgher et al .,2013; Patel and Gupte, 2014).

dyeing wastewaters.

Various studies of ligninolytic enzymes mediated dye

Different physico-chemical methods are available for the

decolorization have been carried out using liquid culture

remediation of dye wastewaters, but, their application is

conditions using homogenized mycelium or pellets of

limited due to some technical and economical drawbacks

white rot fungi (Eichlerova et al ., 2005; Erkurt et al .,

(Ali, 2010). Bioremediation therefore offers a cheaper

2007, Singh et al . 2012).

and environmentally friendlier alternative (Pathak et al .

The present work focuses on isolation and screening of

2011). Many microorganisms have proven significant

synthetic textile dyes decolorizing white rot fungal culture

tools for the development of biological processes for the

AGYP-1. Various physico-chemical parameters like pH,

treatment of textile effluents (Yang et al ., 2009; Chen

temperature, dye concentration, carbon and nitrogen

et al ., 2011; Gahlout et al ., 2013; Shah, 2014). The

source were optimized for the enhanced decolorization

application of lignin-degrading white rot fungi (WRF) has

of Reactive Red M5B. Furthermore, the relationship

attracted increasing scientific attention, as they are able to

between dye decolorization and concomitant ligninolytic

Figure 1: Decolorization of Reactive Red M5B by isolate AGYP-1 on solid medium (A), growth of AGYP-1 on malt extract

agar (B), plate assay for ligninolytic enzyme on SDA plate containing 0.01% orth-dianisidine

500