Molecular study of Pigeonpea

(572.34±24.6 U ml -1 ) and MnP (171±7.2 U ml -1 ) activities

enzyme activities may be owing to highly acidic condition

followed by glucose (91.35±3.5%). Sodium acetate was

or catabolic repression at higher maltose concentration.

found to be poor carbon sources allowing 64.32±3.3%

decolorization, whereas sucrose, lactose, starch, fructose

and xylose supported the decolorization in the range

between 74.32±5.4 and 89.55±2.8% by AGYP-1. Glucose

is known as the most readily usable carbon source for

most of the fungi. However, it is a costly carbon source

and is generally not used in wastewater treatment (Erdal

and Taskin, 2010). With this context, the preference of

maltose as a co-substrate by isolate AGYP-1 offers an

additional advantage for the removal of dye containing

wastewaters. Similar results were reported by Asgher et

al (2013), in which they obtained 91% decolorization of

Solar Brilliant Red 80 by Schizophyllum commune IBL-

Figure 7: Effect of maltose concentration on decolorization

06 in the presence of maltose.

of Reactive Red M5B by fungal isolate AGYP-1

Figure 6: Double reciprocal plot of Reactive Red M5B

Figure 8: Effect of gelatine concentration on decolorization

biodecolorization by fungal isolate AGYP-1

of Reactive Red M5B by fungal isolate AGYP-1

The concentration of maltose in the medium was

varied in the range of 1.0 to 50 g l -1 (w/v) for maximal

3.4.6 Effect of nitrogen source

removal of Reactive red M5B by isolate AGYP-1. The

The decolorization of Reactive Red M5B was performed

decolorization efficiency of the isolate AGYP-1 increased

in the presence of different organic and inorganic

with an increase in maltose concentration from 1.0 to 20

nitrogen sources; maximum decolorization of the dye

g l -1 ; with maximum color removal (96.33±1.2%) and

was obtained with gelatine (96.74±1.7%) after 10 days

laccase (963.38±28.34 U ml -1 ) and MnP (441.63±20.13

(Table 3). The growth of the isolate AGYP-1 was

U m -1 ) activities at 20 g l -1 (Fig. 7). The obtained fungal

0.297±0.02 g along with laccase and MnP activities

growth was 0.277±0.03 g at 20 g l -1 maltose concentration.

of 1537.1±14.6 and 791.64±12.5 U ml -1 respectively.

Any further increase in maltose concentration did not

Following gelatine, yeast extract and urea exhibited

influence an overall decolorization potential suggesting

93.73±4.4 and 92.41±3.5% decolorization of Reactive

20 g l -1 maltose concentration as an optimum for growth

Red M5B. The color removal efficiency of the isolate

and decolorization of the dye. The decolorization of the

was much lower in the presence of inorganic nitrogen

dye was 73.48±2.2% at 50 g l -1 concentration of maltose.

sources. Many researchers have reported enhanced

The reduced decolorization along with lower ligninolytic

decolorization activity of fungal cultures in the presence

507