International Journal of Agriculture, Environment & Biotechnology
Citation: IJAEB: 7(4): 889-894 December 2014
DOI Number: 10.5958/2230-732X.2014.01401.6
©2014 New Delhi Publishers. All rights reserved
In vitro multiplication of madhunashini ( Gymnema sylvestre
Hosahalli Kaganath Akshitha 1 *, Konnan Umesha 2 , Bangalore Narayanappa
Sathyanarayana 3 and Waman Ajit Arun 4
1 Division of Crop Improvement and Biotechnology, Indian Institute of Spices Research, Kozhikode-673 012, Kerala, India
2 Department of PMAS, PG Centre, UHS Campus, GKVK Post, Bangalore, Karnataka-560 065, India
3 Department of Horticulture, UAS, GKVK, Bangalore, Karnataka-560 065, India
4 Division of Horticulture and Forestry, Central Island Agricultural Research Institute (ICAR), Port Blair- 744101, Andaman and Nicobar
Islands, India
*Corresponding author:
Paper No. 282
Received : 10 April, 2014
Accepted: 15 November, 2014
Published: 20 December, 2014
Study was conducted for the in vitro multiplication of madhunashini by using immature seeds as
explants. Immature seeds were cultured directly on to media containing different concentrations and
combinations of auxin and cytokinin. Germination of seeds into plantlets was observed in the media
containing cytokinins, however, the seeds cultured on media containing auxins at the expense of plantlet
formation produced callus. Cent per cent of the explants responded when inoculated on Murashige and
Skoog (MS) media containing Benzylaminopurine (BAP - 1.0 mg l -1 ), Kinetin (Kn - 0.5 mg l -1 , 1.0 mg l -1
and 2.0 mg l -1 ) and BAP + Kn (1.0+1.0 mg l -1 ). Height of plantlet (19.74 mm) was significantly higher in
MS media supplemented with BAP 0.5 mg l -1 . Maximum number of leaves (3.12) were produced on MS
media supplemented with Kn 2.0 mg l -1 . Nodal explants obtained from in vitro seedlings when cultured
on the media containing cytokinins induced proliferation of shoots. Number of shoots (5.50) produced
and length of shoot (18.53 mm) was found to be maximum at lower concentrations of BAP 1.0 mg l -1
and Kn 0.5 mg l -1 . Leaves from in vitro seedlings when used as explants and cultured on media with
cytokinins did not show any response.
  • This study is the first study in madhunashini on the use of immature seeds as explants for in vitro
  • culture
  • Immature seeds responded well in Murashige and Skoog media
  • Rooting was confirmed by using fluorescent microscopy
  • Keywords: Growth regulators, immature seeds, nodal explants, murashige and skoog media
    Madhunashini ( Gymnema sylvestre Retz.) is a member
    medicine. In India, it has been known from antiquity
    of the family Asclepiadaceae and indigenous to
    for the treatment of Diabetes mellitus.
    India. It is called Gudmar in Hindi and madhunashini
    The data on the exact area and production of this
    in Kannada and Sanskrit, means sugar or sweetness
    crop is not available and due to heavy demand, the
    destroyer. It is used both in the Ayurvedic and
    plant has become endangered and its commercial
    Unani medicine. The plant is stomachic, laxative and
    cultivation has recently gained importance in
    diuretic. It is used both in the Ayurvedic and Unani