International Journal of Agriculture, Environment & Biotechnology
Citation: IJAEB: 7(4): 759-764 December 2014
DOI Number: 10.5958/2230-732X.2014.01384.9
©2014 New Delhi Publishers. All rights reserved
Influence of ethylene inhibitor on shoot organogenesis and
regeneration in sugarcane ( Saccharum spp. hybrid)
P. Manchanda*, A. Kaur, K. S. Thind 1 and S. S. Gosal 2
1 Regional Research Station, Kapurthala, Punjab, India.
2 Directorate of Research, School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab-141 004, India.
Corresponding author:
Paper No. 265
Received: 18 August, 2014
Accepted: 15 October, 2014
Published: 17 December, 2014
Ethylene is a gaseous plant hormone (phytohormone) that play an important role on in vitro shoot
morphogenesis in many plant species. The aim of the present investigation was to critically analyse
the role of an ethylene inhibitor i.e. silver nitrate (AgNO 3 ) on shoot organogenesis in sugarcane cvs.
CoJ 83 and CoH 119. Different concentrations of silver nitrate were tested in the medium: 1 mgl -1 ,
3 mgl -1 , 5 mgl -1 and 10 mgl -1 . Genotypic dependence was observed between cvs. CoJ 83 and CoH 119
with different concentrations of AgNO 3 tested in the medium. Among the varying concentrations
(1–10 mgl -1 ) of silver nitrate tested, per centage of leaf roll segments responding were improved giving
highest number of shoots per explant on MS medium + Naphthalene Acetic Acid (NAA; 5 mgl -1 ) +
Kinetin (Kin; 0.5 mgl -1 ) + AgNO 3 (3 mgl -1 ) in cv. CoJ 83 and on MS medium + NAA (5.5 mgl -1 ) + Kin
(0.5 mgl -1 ) + AgNO 3 (5 mgl -1 ). The number of shoots formed per explant increased to two-fold and the
shoot length was also increased as compared to control. Data recorded on the number of roots and root
length also showed a significant increase over the control in both the varieties.
  • A ubiquitous hormone, ethylene (C 2 H 4 ), is produced which is associated with poor regeneration
  • and recalcitrant behaviour of culture material during plant tissue culture experiments
  • In order to study its effect, ethylene production was inhibited in the culture bottles using silver
  • nitrate (AgNO 3 )
  • In two commercial cultivars of sugarcane, a significant enhancement in the per cent establishment
  • of cultures, number of shoots formed per explant, length of shoots, number of roots and root length
    was observed
  • Thus, silver nitrate can be included as an ethylene inhibitor in the media used for the tissue culture
  • studies in sugarcane
    Keywords: Shoot morphogenesis, silver nitrate, ethylene, genotypic dependence
    Sugarcane, one of the most efficient biofuel crops in
    occupies a unique position in agro-industrial
    the world, belongs to genus Saccharum and family
    market by providing raw materials to produce
    Gramineae . India is the largest producer of cane sugar
    sugar, jaggery and khandsari and producing co-
    and second largest producer of cane next to Brazil.
    products like alcohol, paper, cattle feed, electricity
    It is a widely cultivated crop in the subtropical belt
    etc. Nowadays, tissue culture is widely used in
    across North India from Assam to Punjab. Sugarcane
    sugarcane improvement programmes. A large