Influence of ethylene inhibitor on shoot organogenesis

A number of studies have revealed that silver nitrate

of School of Agricultural Biotechnology, Punjab

is known to enhance regeneration in many plants

Agricultural University, Ludhiana. After peeling

like Vitexnegundo (Steephen et al. 2010), Decalepis

of the outer mature leaves, shoot tops (spindles)

hamiltonii (Bais et al. 2000), Cucumismelo (Roustan et

were thoroughly washed with detergent ‘Teepol’

al. 1992). AgNO ₃ has also been reported to enhance in

and then surface sterilized under a laminar air flow

vitro shoot formation in Albizzia julibrissin (Sankhla

cabinet with 1% carbendazim (bavistin) to avoid

et al. 1995), reported microspore embryogenesis in

fungal contamination followed by their sterilization

Brassica juncea (Prem et al. 2005), higher frequency

with 0.1% mercuric chloride for 8-10 minutes to

of multiple shoot formation in Arachis hypogea

avoid bacterial contamination with gentle shaking

(Ozudogru et al. 2005) and plant regeneration in

and rinsed thrice with autoclaved distilled water.

Paspalum scrobiculatum (Kothari-Chajer et al. 2008).

Afterwards,thespindlesweretreatedwithcefotaxime

In this investigation, we report the effects of an

(500 mgl -1 ) for half an hour. Further, preparation

ethylene inhibitor, silver nitrate (AgNO ₃ ) on in vitro

of explants was done from the sterilized spindles.

regeneration in two different cvs. CoJ 83 and CoH

After removing the outer 2-3 leaf whorls and

119 of sugarcane.

the nodal portions from the spindles, innermost

Materials and Methods

leaf whorls were cut transversely into 1 cm long

segments. Inoculations were performed under

The investigation was carried out in Tissue

aseptic conditions. All the instruments (petridish,

Culture and Genetic Transformation laboratories

scalpel and forcep) were sterilized with spirit

at School of Agricultural Biotechnology, Punjab

and heated over flame before use. For shoot

Agricultural University, Ludhiana during 2011-

organogenesis, leaf roll segments were placed on MS

13. Shoot tops were collected from 6-8 month old

medium supplemented with ᾳ-Naphthalene Acetic

field-grown, healthy sugarcane plants grown at

Acid (NAA) [5.0 mgl -1 for CoJ 83 and 5.5 mgl -1 for

University Seed Farm, Laddowal and from the field

CoH 119] supplemented with Kinetin (0.5 mgl -1 ).

Table 1. Effect of different concentrations of silver nitrate on number of shoots, shoot length (cm), number of roots and root

length (cm) in two cvs. of sugarcane (CoJ 83 and CoH 119)

Number of shoots

Shoot length (cm)

Number of roots

Root length (cm)

Variety

CoJ 83

CoH 119

CoJ 83

CoH 119

CoJ 83

CoH 119

CoJ 83

CoH 119

AgNO ₃ (mgl -1 )

Control

6.23*

5.06

4.31

5.20

6.63

6.86

1.56

1.37

1

6.56

5.43

4.49

5.30

6.90

7.19

1.67

1.76

3

7.0

5.62

4.65

5.36

7.13

7.36

2.0

1.90

5

5.63

6.25

4.16

5.69

6.20

7.76

1.16

2.27

10

4.76

5.06

3.97

5.04

5.90

6.70

0.923

1.30

Mean

6.04

5.48

4.32

5.32

6.55

7.17

1.46

1.72

CD (5%)

0.346

0.547

0.104

0.164

0.228

0.360

0.239

0.377

CD

(5%)

0.774

0.232

0.509

0.534

[CoJ83 X CoH119]

*All the values represent means of 15 cultures with 3 replications

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