Influence of ethylene inhibitor on shoot organogenesis
Vanilla planifolia (Giridhar et al. 2001). A similar study
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Chae, S.C. and Park, S.U. 2012. Improved shoot organogenesis
inhibitors
[aminoethoxyvinylglycine
(AVG),
of Echinaceaangustifolia DC treated with ethylene inhibitors.
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cobalt chloride (CoCl 2 ) and silver thiosulphate
(STS)] enhanced shoot organogenesis of Gloxinia
Cheema, H.S. and Singh, B. 1990. A user’s manual to CPCS-
1, A Computer Programme Package for the Analysis of
( Sinningiaspeciosa ). By using AVG and CoCl 2 in the
commonly used experimental designs. Pp. 2-8 Punjab
culture medium at a concentration of 1 mgl -1 , an
Agricultural University, Ludhiana
increase of 16% and 12% in the shoot number was
Fei, S, R., Paul E. and Riordan, T.P. 2000. Improvement of
observed, respectively.
embryogenic callus induction and shoot regeneration of
Rooting of shoots was carried out on liquid MS
buffalograss by silver nitrate. Plant Cell Tissue and Organ
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medium + NAA (3.0 mgl -1 ) + IBA (2.0 mgl -1 ) + sucrose
Fitch, M.M.M. and Moore, P.H. 1990. Long term culture of
(7%) (Figure 1E). Rooting of shoots earlier placed on
embryogenic sugarcane callus. Plant Cell Tissue and Organ
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Culture 32 : 335 - 343
in CoJ 83 and 5 mgl -1 AgNO 3 in CoH 119 was also
Giridhar, P., Obul Redy B. and Ravishankar, G.A. 2001. Silver
improved with respect to number of roots and root
nitrate influences in vitro shoot multiplication and root
length. Significant variation was observed within the
formation in Vanilla planifolia Andr. Current Science 81 : 1170
varieties as well as between the varieties. Average
Kothari-Chajer, A., Sharma, M., Kachhwaha S., and Kothari,
number of roots was increased to 7.13 from 6.63 in
S.L., 2008. Micronutrient optimization results into highly
improved in vitro plant regeneration in kodo ( Paspalum
control with an average root length of 2.0 cm in CoJ
scrobiculatum L.) and finger ( Eleusine coracana (L.) Gaertn.)
83 and increased to 7.76 from 6.86 (control) with an
millets. Plant Cell Tissue and Organ Culture 94 (6): 105-112.
average root length of 2.27 cm in CoH 119 (Table 1).
Mittal, P., R. Devi and S.S. Gosal. 2013. Direct plant
Plantlets with well-developed root and shoot system
regenerationfrom Spindle Leaf Roll Explants in Sugarcane.
were hardened on pre-soaked cotton under high light
Indian Journal of Ecology 40 : 67-70
intensity (5000 lux) for 4 days in test tubes under in
Ozudogru, E., Y. Ozden Tokatli and A. Akcin. 2005. Effect
vitro conditions (Figure 1F). The hardened plantlets
of silver nitrate on multiple shoot formation of Virginia-
type peanut through shoot tip culture. In vitro cellular and
were transferred to the soil with farmyard manure in
Development Biology-Plant 41 : 151-156.
the polybags (Figure 1G). The results reported in the
Prem, D.; K. Gupta and A. Agnihotri. 2005. Effect of various
present investigation showed that in two commercial
exogenous and endogenous factors on microspore
cultivars of sugarcane, a significant enhancement in
embryogenesis in indian mustard ( Brassica juncea (L.)
the per cent establishment of cultures, number of
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shoots formed per explant, length of shoots, number
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of roots and root length was observed. Therefore,
Parthasarathy, N. 1948. Origin of North Indian Sugar Cane
( Saccharum officinarum L.). Nature 161 : 608
it is concluded that, as an efficient regeneration
Pua, E.C. and Chi, G.L., 1993. De novo shoot morphogenesis
system is essential for mass multiplication and
and plant growth of mustard ( Brassica juncea ) in vitro in
genetic transformation studies, silver nitrate can be
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Purnhauser, L., Medgysey P., Czako, M. Dix J.P. and Marton,
for tissue culture studies in sugarcane (Saccharum
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aestivum and Nicotiana plumbaginifolia Viv tissue cultures
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