In vitro evaluation of plant growth regulators on tissue
Table 2. Production of plant growth regulators by isolates of fluorescent Pseudomonas species
Plant growth regulators ( µg /ml)
Isolates
Auxins *
Gibberellins**
Cytokinins ***
An-1-Kul
14.72
485.8
172.7
An-2-Kul
7.27
252.2
90.9
An-3-Kul
6.94
203.5
154.5
An-4-Kul
6.00
196.4
81.8
An-5-Kul
5.83
303.5
90.9
An-6-Kul
5.11
205.8
45.4
An-7-Kul
6.00
316.1
45.4
An-8-Kul
21.00
160.0
140.9
An-9-Kul
8.22
143.5
45.4
An-10-Kul
5.50
165.4
77.2
An-11-Kul
9.27
261.2
90.9
An-12-Kul
16.11
174.8
90.9
An-13-Kul
16.92
233.2
90.9
An-14-Kul
10.22
275.4
113.6
An-15-Kul
5.61
324.1
68.1
An-16-Kul
6.94
292.9
63.6
An-17-Kul
7.61
207.7
63.6
An-18-Kul
5.16
299.6
181.8
An-19-Kul
8.55
212.5
90.9
Ar-1-kul
4.77
267.3
136.3
Ar-2-kul
7.33
232.2
181.8
Ar-3-kul
10.43
361.1
227.2
Ar-4-kul
5.88
299.9
90.9
Pn-1-Kul
12.98
419.2
295.4
Pn-2-Kul
1.83
116.1
186.3
Pn-3-Kul
12.76
419.2
249.9
Pn-4-Kul
3.38
251.5
113.6
Pn-5-Kul
2.71
309.6
45.4
Pr-1-Kul
3.60
180.6
68.1
Pr-2-Kul
3.38
254.7
81.8
CD .05
3.19
16.41
3.23
Extracellular Production of plant growth regulators i.e.Auxins*, Gibberellins** and Cytokinins*** expressed in terms of concentration
(µg/ml) in 72h old supernatant as calibrated from the standard curve of indole acetic acid(IAA); 10-100(µg/ml) , gibberellic acid
(GA 3 ) ;100-1000(µg/ml),kinetin;100-1000 (µg/ml)
16S rDNA gene was amplified by PCR using the set
PCR amplification was done as follow: denaturation
of primers FP-1(GGTCTGAGAGGATGATCAGT)
at 94°C for 1 min, annealing at 55°C and elongation
and RP-1(TTAGCTCCACCTCGCGGC) in MJ Mini
at 72°C for 2 min; with a total of 35 cycles (Widmer et
BIO-RAD personal thermal cycler-100 (PTC-100).
al., 1998; Weisburg, et al., 1991). For DNA sequencing,
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