In vitro evaluation of plant growth regulators on tissue
Table 3. Effect of different concentrations of partial purified cytokinins from An-1-kul on callus formation, %shoot
regeneration and average shoots per explants from cotyledons of broccoli ( Brassica oleracea L. var. italica ) explant
Treatments
Callus formation
**Shoots
Callus
*% Shooting
S. No.
(column purified
Replications
Post
Incre.
per
cytokinins)
Pre wt.
Conc. formation regeneration
wt.
wt.
explants
CK 0 1
0.05
0.15
0.10
261.0
+
-
-
CK 0 2
0.06
0.19
0.13
341.0
+
-
-
0.04
0.15
0.11
281.0
+
-
-
1.
CK (control)
CK 0 3
0
CK 4
0
0.00
0.00
0.00
0.00
-
-
-
CK 0 5
0.00
0.00
0.00
0.00
-
-
-
CK 0 6
0.00
0.00
0.00
0.00
-
-
-
CD (0.05)
1.26
CK 1 (1.0mg/l)-1
0.11
0.18
0.07
180.0
+
-
-
CK 1 (1.0mg/l)-2
0.098
0.23
0.13
340.0
+
-
-
0.09
0.26
0.17
440.0
+
-
-
CK1(1.0mg/l)
CK 1 (1.0mg/l)-3
2.
CK 1 (1.0mg/l)-4
0.08
0.19
0.11
280.0
+
-
-
CK 1 (1.0mg/l)-5
0.12
0.26
0.14
360.0
+
-
-
CK 1 (1.0mg/l)-6
0.00
0.00
0.00
0.00
-
-
-
CD (0.05)
1.62
CK 1 (1.5mg/l)-1
0.10
0.20
0.10
260.0
+
-
-
CK 1 (1.5mg/l)-2
0.10
0.33
0.23
600.0
+
-
-
0.15
0.39
0.24
630.0
+
-
-
CK (1.5mg/l
CK 1 (1.5mg/l)-3
1
CK (1.5mg/l)-4
1
0.08
0.21
0.13
340.0
+
-
-
CK 1 (1.5mg/l)-5
0.08
0.27
0.19
500.0
+
-
-
3.
CK 1 (1.5mg/l)-6
0.08
0.35
0.27
710.0
+
-
-
CD (0.05)
1.78
CK 1 (2.0mg/l)-1
0.07
0.24
0.17
440.0
+
¼ x100=25
3/4
4.
CK (2.0mg/l)-2
1
0.1
0.15
0.05
120.0
+
2/20 x100=10
6/20
0.10
0.18
0.08
200.0
+
-
-
CK (2.0mg/l
CK 1 (2.0mg/l)-3
1
CK (2.0mg/l)-4
1
0.03
0.23
0.20
520.0
+
-
-
CK 1 (2.0mg/l)-5
0.04
0.09
0.05
120.0
+
-
-
CK 1 (2.0mg/l)-6
0.03
0.08
0.05
120.0
+
-
-
CD (0.05)
1.78
aeruginosa DK2 (Accession number NC_018080.1)
Accession number
and 99 % similarity with Pseudomonas aeruginosa PA7
The sequences were submitted to GenBank and
(Accession number NC_009656.1) respectively to the
accession numbers were assigned for isolate
sequences within GenBank.
KF682468, KF682469 to An-1-kul and An-13-kul
respectively.
753