Sharma et al.
Table 4. Effect of different concentrations of partial purified cytokinins from An-13-kul on callus formation, %shoot
regeneration and average shoots per explants from cotyledons of broccoli ( Brassica oleracea L. var. italica ) explant
Treatments
Callus formation
Shoots
Callus
% Shooting
S. No. (column purified
Replications
Incr.
per
formation
regeneration
cytokinins)
Pre wt. Post wt.
Conc.
wt.
explants
CK 0 1
0.05
0.15
0.10
261.0
+
-
-
CK 0 2
0.06
0.19
0.13
341.0
+
-
-
CK 0 3
0.04
0.15
0.11
281.0
+
-
-
1.
CK (control)
0
CK 4
0
0.00
0.00
0.00
0.00
-
-
-
CK 0 5
0.00
0.00
0.00
0.00
-
-
-
CK 0 6
0.00
0.00
0.00
0.00
-
-
-
CD (0.05)
1.26
CK 13 (1.0mg/l)-1
0.04
0.19
0.15
390.0
+
-
-
CK 13 (1.0mg/l)-2
0.02
0.18
0.16
420.0
+
6/4 x 100 =150 2/4
CK 13 (1.0mg/l)-3
0.11
0.23
0.12
300.0
+
-
-
CK (1.0mg/l)
2.
13
CK 13 (1.0mg/l)-4
0.09
0.28
0.19
500.0
+
-
-
CK 13 (1.0mg/l)-5
0.07
0.165
0.095
240.0
+
-
-
CK 13 (1.0mg/l)-6
0.11
0.25
0.14
360.0
+
-
-
CD (0.05)
1.779
CK 13 (1.5mg/l)-1
0.12
0.30
0.18
470.0
+
-
-
CK 13 (1.5mg/l)-2
0.10
0.19
0.09
180.0
+
-
-
CK 13 (1.5mg/l)-3
0.04
0.14
0.10
260.0
+
-
-
CK (1.5mg/l
13
CK (1.5mg/l)-4
13
0.10
0.30
0.20
520.0
+
-
-
CK 13 (1.5mg/l)-5
0.12
0.21
0.09
230.0
+
-
-
CK 13 (1.5mg/l)-6
0.11
0.25
0.14
360.0
+
-
-
3.
CD (0.05)
1.779
4.
CK 13 (2.0mg/l)-1
0.00
0.00
0.00
0.00
-
-
-
CK 13 (2.0mg/l)-2
0.00
0.00
0.00
0.00
-
-
-
CK 13 (2.0mg/l)-3
0.00
0.00
0.00
0.00
-
-
-
CK (2.0mg/l)
13
CK (2.0mg/l)-4
13
0.135
0.25
0.12
300.00
-
-
-
CK 13 (2.0mg/l)-5
0.00
0.00
0.00
0.00
-
-
-
CK 13 (2.0mg/l)-6
0.14
0.29
0.15
390.00
-
-
-
CD (0.05)
1.026
Effect of partially purified cytokinins from
growth regulators and to study mechanism by which
Pseudomonas aeruginosa An-1-kul and An-13-kul
PGPR stimulate plant growth and callus formation.
on callus formation and shoots regeneration in
The results showed different concentrations of
broccoli ( Brassica oleracea L. var. italice) cotyledon
partially purified cytokinins from Pseudomonas
explants
aeruginosa An-1-kul and An-13-kul were effective in
The aim of the study was to use and to standardize
inducing callus formation and shoot regeneration in
tissue culture based bioassays for (PGPR) plant
cotyledon explants of broccoli. The maximum effect
754