Rajput et al.
of Acacia nilotica were used as study material for this
Application of saline solution
study. The seeds were collected during May-June
of year 2003-05 from different districts of Haryana,
In present study, the seeds were scarified using a
M.P., U.P., Rajasthan, Chhattisgarh and Maharastra
sterilizedfingernailcuttertoremoveaminuteportion
states. The name of districts, soil conditions, and
of seed coat of each seed from the cotyledon end
their longitude, latitude and altitude (above mean
(ISTA, 1987; Dantu et al., 1992). For each treatment 10
sea level) have been given in Table 1.
seeds per replication and a total of three replications
were used in present study. Thus, a total of 30 seeds
Preparation of Saline solutions
per treatment of each provenance and 180 seeds of
The saline solutions were prepared by using the
each provenance for all the six treatments were used.
For 15 provenances a total of 2700 seeds were used.
different amount of NaCl, Na 2 SO , NaHCO , CaCl
4
3
2
The germination was tested using petri dishes in
and MgSO 4 to get electrical conductivity of solution
as 3, 6, 9, 12 and 15 dSm -1 (Table 2). For comparing
laboratory as well as poly bags in nursery. Before
the saline solution of different concentration one
putting the seed in petri dishes all the scarified seeds
control (OEC solution) was also used. In this way,
were soaked in the solution of different EC (0 to 15)
six level of saline solution was prepared and their
for 24 hours. In case of control the seeds were soaked
real electrical conductivity and pH was observed to
in distilled water. After soaking the seed in saline
know the exempt concentration of solution (Table 3).
Table 1. Geographical and soil characteristics of the provenances of Acacia nilotica
S.N.
Provenance
State
Latitude (N) Longitude (E) Altitude (m)
Soil type
1.
Sagar
M.P.
78° 43’
23° 51’
532
Clay loam, black
2.
Jabalpur
M.P.
79° 56’
23° 10’
394
Loam, Clay loam, black
3.
Raipur
Chhattishgarh
81° 37’
21° 15’
325
Clay loam, black and sandy
4.
Nagpur
Maharashtra
79° 56’
23° 10’
394
Sandy brown and Clay black
5.
Akola
Maharashtra
81° 37’
21° 15’
325
Sandy brown and Clay black
6.
Khandwa
M.P.
79° 05’
21° 09’
304
Grayish and black Clay
7.
Ujjain
M.P.
76° 58’
20° 42’
297
Black Clay loam
8.
Shajapur
M.P.
76° 22’
21° 48’
303
Sandy brown and Clay black
9.
Bhopal
M.P.
77° 24 ’
23° 15’
545
Clay loam, red and Black
10.
Guna
M.P.
77° 18’
24° 38’
487
Clay loam, Red sandy
11.
Jhansi
U.P.
78° 32’
25° 30’
271
Black and Red sandy
12.
Bharatpur
Rajasthan
75° 47’
27° 07’
432
Sandy loam and fine loam
13.
Jaipur
Rajasthan
77° 29’
27° 13’
175
Sandy loam and fine loam
14.
Rewari
Haryana
76° 34’
28° 53’
224
Sandy loam
15.
Karnal
Haryana
77° 02’
29° 42’
232
Clay loam
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