Priyadarsini et al.
Table. 2. Effect of different concentrations of Fe on the
Var. Khandagiri
growth of callus of O.sativa Var. Khandagiri and Jagannath
( T o l e r a n t
cultured on MS medium supplemented with 1.0 mg/l Kn,
callus)
3.0 mg/l 2,4-D and 3% sucrose after four weeks of culture.
1.5 (Control)
2.62 ± 0.8
1.96 ± 0.3
4.58 ± 1.1 c
Fe Concentration
Growth Tolerance Index (Mean ±
2.87 ± 0.7 2.16 ± 0.6 5.03 ± 1.3 e
4.0
(mM)
SE)*
(+9.54)
(+10.2)
(+9.82)
Var. Khandagiri
Var. Jagannath
3.15 ± 0.6 2.62 ± 0.8 5.77 ± 1.4 i
8.0
1.5 (Control)
74.5 ± 1.6 f
68.8 ± 1.4 e
(+20.2)
(+33.7)
(+25.9)
4.0 mM
68.6 ± 1.8 e
60.2 ± 1.2 d
3.89 ± 0.8 2.78 ± 0.2 6.67 ± 1.0 j
10.0
8.0 mM
62.4 ± 1.2 d
59.4 ± 1.3 d
(+48.5)
(+41.8)
(+45.6)
10.0 mM
56.8 ± 0.9 c
52.2 ± 1.1c
4.16 ± 0.4 3.56 ± 0.5 7.72 ± 0.9 n
12.0
(+58.8)
(+81.6)
(+68.5)
12.0 mM
50.6 ± 0.8 b
48.6 ± 1.5 b
5.24 ± 0.5 3.72 ± 0.6 8.96 ± 1.1 q
14.0 mM
24.4 ± 0.7 a
± 1.2 a
14.0
(+100.0)
(+89.8)
(+95.6)
*Data in parentheses indicate the percentage of reduction (-)
Var. Jagannath
/ increase (+) relative to control. 15 replicate per treatment;
(Non-tolerant
repeated three times.
callus)
a-f Meanshavingthesameletterinacolumnwerenotsignificantly
1.5 (Control)
2.06 ± 0.6
1.80 ± 0.5
3.91 ± 1.1 a
different by the post-hoc multiple comparison tests, P<0.05.
2.45 ± 0.4 1.85 ± 0.6 4.37 ± 1.0 b
4.0
(+18.9)
(+ 2.77)
(+11.4)
Table 3. Chlorophyll content (mg/g fresh weight basis)
3.12 ± 0.3 2.34 ± 0.7 5.46 ± 1.0 g
8.0
of tolerant and non-tolerant callus of O.sativa Var.
(+51.4)
(+30.0)
(+41.5)
Khandagiri and Var. Jagannath cultured on MS medium
3.45 ± 0.7 2.51 ± 0.8 5.96 ± 1.5 k
10.0
supplemented with 1.0 mg/l Kn, 3.0 mg/l 2,4-D and different
(+67.5)
(+39.4)
(+54.4)
concentrations of Fe after four weeks of culture.
4.14 ± 0.8 3.28 ± 0.5 7.42 ± 1.3 m
12.0
(+100.9)
(+82.2)
(+92.2)
Chlorophyll content (mg/g fresh weight)
4.26 ± 0.7 3.68 ± 0.4 7.94 ± 1.1 o
Metal
of callus (Mean ± SE)*
14.0
(+106.8)
9101.6)
(+105.7)
concentration
Total
(mM)
Chloro-
Chloro-
chlorophyll
Var. Jagannath
phyll-a
phyll-b
(a + b)
( T o l e r a n t
Var.
callus)
Khandagiri
1.5 (Control)
2.08 ± 0.4
2.13 ± 0.5
4.21 ± 0.9 b
(Non-tolerant
2.45 ± 0.5
2.22 ± 0.6
4.67 ± 1.1 d
callus)
4.0
(+17.8)
(+4.22)
(+10.92)
1.5 (Control)
2.21 ± 0.5
1.72 ± 0.4
3.93 ± 0.9 a
2.67 ± 0.6
2.89 ± 0.7
5.56 ± 1.3 h
8.0
2.87 ± 0.7 (+ 1.89 ± 0.6
4.76 ± 1.3 d (+
(+28.4)
(+35.7)
(+32.06)
4.0
29.7)
(+9.90)
21.1)
3.82 ± 0.7
3.24 ± 0.4
7.06 ± 1.1 l
10.0
3.12 ± 0.4 (+ 2.22 ± 0.7
5.34 ± 1.1 f
(+83.6)
(+52.1)
(+67.7)
8.0
41.2)
(+29.0)
(+35.9)
4.53 ± 0.4
3.58 ± 0.6
8.11 ± 1.0 p
12.0
3.25 ± 0.5 (+ 2.36 ± 0.4
5.61 ± 0.9 h
(+117.8)
(+68.0)
(+92.6)
10.0
47.0)
(+37.2)
(+42.7)
4.92 ± 0.5
3.94 ± 0.5
8.86 ± 1.0 q
14.0
4.16 ± 0.7
2.78 ± 0.3
6.94 ± 1.0 k
(+136.5)
(+84.9)
(+110.4)
12.0
(+88.2)
(+61.6)
(+76.6)
*Four replicates per treatment; repeated three times.
4.86 ± 0.6
3.14 ± 0.6
8.00 ± 1.2 p
14.0
Data in parenthesis indicate the percentage of reduction (-)/
(+119.9)
(+82.5)
(+103.6)
increase (+) relative to control.
a – q Means having the same letter within a column were not
significantly different by the post-hoc multiple comparison tests,
p< 0.05.
288