Study of in vitro selection and plant regeneration of Indica rice tolerant to iron
Table 4. Total protein content (µg/g fresh weight basis) of
10.0
40.8 ± 1.0 (+68.6) 32.8 ± 0.6 (+38.9)
tolerant and non-tolerant callus of O.sativa Var. Khandagiri
and Var. Jagannath cultured on MS medium supplemented
12.0
45.2 ± 1.3 (+86.8) 24.8 ± 0.9 (+5.08)
with 1.0 mg/l Kinetin and 3.0 mg/l 2,4-D and different
14.0
53.6 ± 1.1 (+121.5) 19.56 ± 0.8 (-17.1)
concentrations of Fe after four weeks of culture.
Var. Jagannath
1.5 (Control)
21.8 ± 1.0
22.2 ± 0.7
Metal
concentration
Source of callus
4.0
25.6 ± 1.2 (+17.4) 26.6 ± 0.9 (+19.8)
(mM)
8.0
28.4 ± 1.4 (+30.3) 29.6 ± 0.8 (+33.3)
Non-tolerant
10.0
30.6 ± 1.2 (+40.4) 24.2 ± 0.7 (+9.00)
Tolerant callus
callus
12.0
36.8 ± 1.3 (+68.8) 11.4 ± 0.9 (- 17.11)
Var. Khandagiri
14.0
42.8 ± 1.4 (+96.3) 8.34 ± 0.6 (- 48.9)
1.5 (Control)
2.79 ± 0.8 a
2.90 ± 0.5 c
*Data represent in parenthesis indicate the percentage of
4.0
3.24 ± 0.6 b (+16.1)
2.96 ± 0.4 c (+2.0)
reduction (-) / increase (+) relative to control. Four replicates per
8.0
3.88 ± 0.7 d (+39.0)
3.11 ± 0.7 d (+21.0)
treatment; repeated three times.
10.0
4.42 ± 0.6 e (+58.4)
3.32 ± 0.8 e (+14.5)
12.0
4.68 ± 0.8 f (+67.7)
3.84 ± 0.4 f (+32.4)
Table. 6. Peroxidase activity of toloerant and non-tolerant
14.0
5.12 ± 0.5 h (+83.5)
4.05 ± 0.5 g (+39.6)
callus of O.sativa Var. Khandagiri and Var. Jagannath
grown on MS medium supplemented with 1.0 mg/l Kinetin
Var. Jagannath
and 3.0 mg/l 2,4-D and different concentrations of Fe after
four weeks of culture.
1.5 (Control)
2.82 ± 0.5 a
2.12 ± 0.5 a
4.0
3.56 ± 0.6 c (+26.2)
2.25 ± 0.6 a (+6.13)
Peroxidase activity (µmol H 2 O 2
8.0
3.92 ± 0.7 d (+39.0)
2.58 ± 0.6 b (+21.7)
Variety
destroyed/min/mg protein)
10.0
4.77 ± 0.5 g (+69.1)
2.96 ± 0.8 c (+39.6)
Tolerant
Non-tolerant
Var. Khandagiri
12.0
5.23 ± 0.6 h (+85.5)
3.01 ± 0.9 d (+41.9)
1.5 (Control)
12.6 ± 0.8
11.8 ± 0.8
5.66 ± 0.4 i (+100.7)
3.24 ± 0.7 e (+52.8)
4.0 mM
16.2 ± 1.1 (+28.6)
14.6 ± 0.7 (+23.7)
*Data in parenthesis indicate the percentage of reduction (-)/
8.0 mM
23.4 ± 0.9 (+85.7)
18.2 ± 0.6 (+54.2)
increase (+) relative to control. Four replicates/ treatment;
repeated thrice.
10.0 mM
28.8 ± 0.8 (+128.6)
22.4 ± 0.5 (+72.9)
a- i Means having the same letter within a column were not
12.0 mM
32.6 ± 0.7 (+158.7)
17.8 ± 0.7 (+50.8)
significantly different by the post-hoc multiple comparison tests,
14.0 mM
42.8 ± 0.8 (+239.7)
10.1 ± 0.6 (- 14.4)
p<0.05 level.
Var. Jagannath
1.5 (Control)
14.2 ± 0.8
14.6 ± 0.6
Table. 5. Catalase activity of toloerant and non-tolerant
4.0 mM
20.6 ± 0.6 (+45.07)
18.4 ± 0.5 (+26.0)
callus of O.sativa Var. Khandagiri and Var. Jagannath
8.0 mM
25.6 ± 0.7 (+80.3)
22.5 ± 0.7 (+54.1)
grown on MS medium supplemented with 1.0 mg/l Kinetin
10.0 mM
30.2 ± 0.4 (+112.7)
26.2 ± 0.6 (+65.7)
and 3.0 mg/l 2,4-D and different concentrations of Fe after
12.0 mM
35.8 ± 0.6 (+163.4)
18.4 ± 0.8 (+27.4)
four weeks of culture.
14.0 mM
46.4 ± 0.9 (+226.8)
12.8 ± 0.9 (- 12.3)
Catalase activity (µmol H 2 O 2 destroyed/
*Data represent in parenthesis indicate the percentage of
Variety
min/mg protein)
reduction (-) / increase (+) relative to control. Four replicates per
Tolerant
Non-tolerant
treatment; repeated three times.
Var. Khandagiri
1.5 (Control)
24.2 ± 1.2
23.6 ± 0.9
4.0
28.4 ± 1.0 (+17.3) 26.4 ± 0.8 (+11.9)
8.0
32.4 ± 1.2 (+33.9) 28.6 ± 0.7 (+21.2)
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