Priyadarsini et al.
cultivarsȱ displayȱ superiorȱ performanceȱ underȱ Fe-
showingȱ secondaryȱ callusingȱ (Figureȱ 1D).ȱ Rootedȱ
toxicȱconditionȱpartlyȱdueȱtoȱFeȱtolerance.
plantletsȱwereȱtransferredȱtoȱpotsȱcontainingȱaȱpotȱ
comprisedȱofȱcowȱdung,ȱsandȱandȱiron-richȱsoilȱinȱ
Enzyme activity
theȱratioȱofȱ1:1;1ȱ(v/v),ȱrespectively,ȱandȱkeptȱinȱtheȱ
Theȱenzymeȱactivityȱwasȱexaminedȱinȱbothȱtolerantȱ
greenhouseȱforȱacclimatizationȱ(Figureȱ1E).ȱAfterȱoneȱ
andȱnon-tolerantȱcalluses.ȱTheȱenzymeȱactivitiesȱareȱ
monthȱofȱtransferȱtoȱtheȱsoilȱmixture,ȱaboutȱ70%ȱofȱ
enhancedȱforȱplantȱmetabolismȱunderȱstressȱconditionȱ
theȱplantletsȱsurvivedȱinȱtheȱgreenhouse.ȱTheȱplantsȱ
and,ȱ therefore,ȱ mayȱ playȱ aȱ roleȱ inȱ metalȱ toleranceȱ
areȱbearingȱpaniclesȱafterȱ60ȱdaysȱofȱtransferȱtoȱtheȱ
(VanȱAsscheȱandȱClijstersȱ1990).ȱDuringȱtheȱgrowthȱ
potȱ(Figureȱ1F).
period,ȱtheȱactivitiesȱofȱbothȱcatalaseȱandȱperoxidaseȱ
Inȱ Conclusion,ȱ theȱ tolerantȱ callusȱ linesȱ showedȱ
areȱsignificantlyȱhigherȱinȱtolerantȱcallusesȱgrownȱinȱ
toleranceȱ toȱ Feȱ andȱ theȱ plantsȱ areȱ alsoȱ growingȱ
theȱmediumȱhavingȱ12ȱmMȱFeȱinȱcomparisonȱwithȱ
normallyȱinȱtheȱironȱrichȱsoil.ȱTheȱenzymeȱactivitiesȱ
non-tolerantȱcallusesȱ(Tablesȱ5ȱandȱ6).ȱHigherȱactivityȱ
wereȱsignificantlyȱhigherȱinȱtolerantȱcallusȱlinesȱandȱ
ofȱcatalaseȱandȱperoxidaseȱindicatedȱthatȱtheȱplantsȱ
pigmentȱ contentȱ wasȱ alsoȱ moreȱ inȱ tolerantȱ callusȱ
derivedȱfromȱtolerantȱcallusesȱwereȱunderȱoxidativeȱ
compareȱ withȱ non-tolerantȱ calluses.ȱ Developmentȱ
stress,ȱaȱfeatureȱoftenȱassociatedȱwithȱmetalȱtolerance.ȱ
ofȱ metalȱ tolerantȱ cellȱ linesȱ mayȱ beȱ usefulȱ inȱ cropȱ
Rout et al. (1999)ȱreportedȱthatȱtheȱbothȱcatalaseȱandȱ
improvementȱ programmes.ȱ Furtherȱ studiesȱ areȱ
peroxidaseȱ activityȱ wereȱ higherȱ inȱ tolerantȱ calliȱ ofȱ
necessaryȱ toȱ identifyȱ theȱ molecularȱ mechanismȱ inȱ
Brassicaȱ junceaȱ andȱ Brassicaȱ campestrisȱ grownȱ onȱ
rice.
0.8mMȱ manganeseȱ andȱ 0.24mMȱ zincȱ containingȱ
growthȱ medium.ȱ Theȱ catalaseȱ andȱ peroxidaseȱ Acknowledgement
activityȱwereȱgenerallyȱhighȱinȱcropsȱgrownȱinȱheavyȱ
metalȱ pollutedȱ soilȱ asȱ reportedȱ earlierȱ (Nashikkarȱ
TheȱauthorsȱwishȱtoȱacknowledgeȱtoȱtheȱDepartmentȱ
andȱ Chakrabarti,1994).ȱ Enzymaticȱ oxidationȱ ofȱ
ofȱ Scienceȱ andȱ Technology,ȱ Govt.ȱ ofȱ Odishaȱ forȱ
Feȱ wasȱ foundȱ toȱ beȱ theȱ principalȱ mechanismȱ forȱ
providingȱresearchȱgrantȱforȱthisȱinvestigation.
higherȱ oxidationȱ activityȱ ofȱ riceȱ rootsȱ andȱ chieflyȱ
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