Patel et al.

ThisȱstudyȱisȱofȱGeneȱexpressionȱofȱ Arachis hypogaea

Transcriptomeȱduringȱseedȱdevelopment,ȱandȱgenerationȱ

L.ȱ andȱ asȱ aȱ partȱ ofȱ research,ȱ threeȱ SRAȱ sequencesȱ

ofȱ genic-SSRȱ markersȱ inȱ Peanutȱ (Arachisȱ hypogaeaȱ L.).ȱ

BMC Genomics 13 :90.ȱhttp://dx.doi.org/10.1186/1471-2164-

SRR1212866,ȱ SRR1212867ȱ andȱ SRR1212868ȱ

13-90

downloadedȱfromȱBioProjectȱIDȱ243319ȱfromȱNCBIȱ

LesleyȱJȱCollins,ȱPatrickȱJȱBiggs,ȱClaudiaȱVoelckelȱandȱSimonȱJolyȱ

database.ȱTheseȱSRAȱfilesȱareȱconvertedȱtoȱ.fastqȱfilesȱ

2008.ȱAnȱapproachȱtoȱtranscriptomeȱanalysisȱofȱnon-modelȱ

byȱ SRAȱ TOOLKITȱ ofȱ NCBI.ȱ Forȱ Geneȱ expressionȱ

organismsȱusingȱshort-readȱsequences.ȱ Genome Informatics

study,ȱ TUXEDOȱ Protocolȱ consideredȱ forȱ analysis,ȱ

21 :3-14.ȱhttp://dx.doi.org/10.1142/9781848163324_0001

inȱwhichȱvariousȱBioinformaticsȱtoolsȱusedȱforȱGeneȱ Marcȱ Libault,ȱ Truptiȱ Joshi,ȱ Vagnerȱ Aȱ Benedito,ȱ Dongȱ Xu,ȱ

expressionȱ studyȱ andȱ CummeRbundȱ toolȱ usedȱ forȱ

Michaelȱ Kȱ Udvardiȱ andȱ Garyȱ Staceyȱ 2009.ȱ Legumeȱ

Transcriptionȱ Factorȱ Genes:ȱ Whatȱ makesȱ legumesȱ soȱ

visualizationȱofȱdata.

special?.ȱ Plant Physiology 151 :ȱ 991-1001.ȱ http://dx.doi.

Theȱ resultsȱ ofȱ thisȱ studyȱ showsȱ thatȱ threeȱ RNA-

org/10.1104/pp.109.144105

Seqȱsamplesȱwithȱtotalȱ27126ȱgenesȱpresentȱinȱthreeȱ Patel,ȱ Raviȱ Kȱ andȱ Jainȱ Mukesh.ȱ 2012.ȱ NGSȱ QCȱ Toolkit:ȱ Aȱ

differentȱ conditionsȱ andȱ variousȱ interestingȱ resultsȱ

ToolkitȱforȱQualityȱControlȱofȱNextȱGenerationȱSequencingȱ

Data.ȱ PLoS ONE 7 (2):e30619.ȱ http://dx.doi.org/10.1371/

obtainedȱfromȱCummeRbundȱtool.ȱThereȱareȱvariousȱ

journal.pone.0030619

statisticalȱresultsȱalsoȱobtainedȱfromȱthisȱstudyȱwhichȱ

RobȱWȱNess,ȱMathieuȱSiolȱandȱSpencerȱCHȱBarrettȱ2011.ȱDeȱ

areȱ discussedȱ inȱ Resultȱ section.ȱ Next-generationȱ

novoȱsequenceȱassemblyȱandȱcharacterizationȱofȱtheȱfloralȱ

sequenceȱdataȱanalysisȱ(NGS)ȱofȱ Arachis hypogaea ȱL.ȱ

transcriptomeȱ inȱ crossȱ andȱ self-fertilizingȱ plants.ȱ BMC

(Theȱpeanut)ȱbasedȱonȱGeneȱexpressionȱstudyȱwillȱ

Genomics 12 :ȱ 298.ȱ http://dx.doi.org/10.1186/1471-2164-12-

298

beȱveryȱusefulȱforȱfurtherȱstudyȱorȱanalysisȱpurpose.

RohiniȱGarg,ȱRaviȱKȱPatel,ȱAkhileshȱKȱTyagi,ȱandȱMukeshȱJainȱ

Acknowledgments

2011.ȱDeȱNovoȱAssemblyȱofȱChickpeaȱTranscriptomeȱUsingȱ

ShortȱReadsȱforȱGeneȱDiscoveryȱandȱMarkerȱIdentification.ȱ

WeȱareȱheartilyȱthankfulȱtoȱProf.ȱ(Dr.)ȱP.V.ȱVirparia,ȱ

DNA Research 18 :ȱ53–63.ȱhttp://dx.doi.org/10.1093/dnares/

Director,ȱ GDCST,ȱ Sardarȱ Patelȱ University,ȱ Vallabhȱ

dsq028

Vidyanagar,ȱ forȱ providingȱ usȱ facilitiesȱ forȱ theȱ

Patel,ȱSagarȱandȱPanchal,ȱHetalȱKumarȱ2014.ȱBioinformaticsȱ

Informationȱ ofȱ Leguminosaeȱ Familyȱ inȱ Gujaratȱ State.ȱ

researchȱwork.

International Journal of Agriculture, Environment and

Biotechnology 7 (1):ȱ 11-15.ȱ http://dx.doi.org/10.5958/j.2230-

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