Int. Jour. of App. Sc. and Eng. (IJASE) 2 (2) : Dec 2014, 83-89
© 2014 New Delhi Publishers. All rights reserved.
1
Phytochemical Analysis and Antimicrobial Activity
of Chlorophytum borivilianum against Bacterial
Pathogen causing disease in Humans
Syed Rehan Ahmad 1 , Abul Kalam 2 and Kishan Pal 3
1,2 Dept. of Microbiology, Bidhannagar Govt College, Sector-1, Salt Lake City, Kolkata, WB-64.
3 Dept of Microbiology, Shri Venkateshwara University, Gajraula, U.P., 244236, India .
Corresponding author : Syed Rehan Ahmad; rehanbiotech1@gmail.com
Abstract
The present communication deals with in vitro analysis of Antibacterial activity of
extract of leaves and stem of Chlorophytum borivilianum Santapau and Fernandez
(Liliaceae). The result of the preliminary investigation revealed the presence of
alkaloids, glycosides nucleus, Saponins and tannins in leaves as well as in stem. The
antimicrobial activity of leaves and stem extract of Chlorophytum borivilianum was
studied against four bacteria among them two are Gram –ve bacteria (Escherichia
coli and Klebsiella pneumonia) and two are Gram +ve positive bacteria ( Staphylococcus
aureus and Bacillus subtilis .) by agar disc cup diffusion method. Zone of inhibition
produced by different extracts was tabulated. Only the aerial parts of plant inhibited
the growth of bacteria at the concentration of 1000mg/ml and 500 mg/ml respectively.
Extract Showed maximum antibacterial activity against all organisms tested in order
of sensitivity as Staphylococcus > Bacillus subtilis > Klebsiella > Escherichia coli .
Keywords: Chlorophytum borivilianum, safed musli, leaves and stem extract,
antibacterial activity, zone of inhibition.
The WHO estimated that 80% of the world’s population depends on traditional medicines
for meeting their primary health care needs[1]. Chlorophytum borivilianum is a wonderful
medicinal herb with sub-erect lanceolate leaves and tuberous root system belonging to the
family Liliaceae. It can grow upto a maximum height of 40 to 47 cm. Tubers can grow upto
a depth of 26 cm. Chorophytum borivilianum is a little annual herb that grows proficiently in
tropical and sub-tropical climates with altitudes upto 1500 meters (Kaushik 2005). In India
17 species of Chlorophytum borivilianum are found and 256 species are found over the home
planet, and out of 17 species, 3 species namely as Chlorophytum borivillianum , Chlorophytum
arundinaceam and Chlorophytum tuberosum are commercially cultivated by the Indian farmers
but Chlorophytum borivillianum is the only species, which is under commercial cultivation.