Int. J. Bioinformatics and Biological Sci.: v.2 n.1&2, p. 23-39. March and June 2014

genetic Diversity analysis and RaPD marker

Inheritance in f ₁ Progenies of mulberry ( Morus spp .)

Jyoti Prakash 1 , v. g. Naik 2 , a. Sarkar 2 and R. K. Salgotra 1*,

1 School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and

Technology of Jammu, Chatha, Jammu-180 009, J and K, INDIA

2 Molecular Biology Laboratory, Central Sericultural Research and Training Institute,

Central Silk Board, Srirampura, Mysore, Karnataka-570 008, INDIA

*Corresponding author: R. K. Salgotra, rks_2959@rediffmail.com

abstract

The present study attempts to understand the pattern of RAPD markers inheritance

in the F ₁ progenies of a cross Mysore Local and V-1 of mulberry ( Morus spp .).

RAPD marker analysis was carried out using 5 decamer random primers. Out of

33 amplicons generated, 20 were of monomorphic and rest 13 were polymorphic

(39:39%). The size of the amplified products ranged from 500-3200 bp. The genetic

distance (GD) between the parents based on RAPD data set is 0.186, which is

low in comparison with morpho-agronomical divergence. In some progenies, GD

calculated was higher than the parents suggesting OPA-02 ₂₉₀₀ , OPA-02 ₉₀₀ , OPA-

02 ₈₀₀

RAPD markers showed expected Mendelian pattern of inheritance. Besides

three other markers, OPA-04 ₁₄₅₀ , OPA-04 ₁₀₀₀

and OPA-07 ₁₀₀₀

showed Mendelian

segregation pattern for 1:1 ratio. The finding proves that the dominant status of the

RAPD markers may not be a limiting factor in genetical analysis of a heterozygous

tree like mulberry, where F ₁ tends to behave like a segregating population. The

polymorphic RAPD markers in parents, segregates as independent alleles in the F ₁

progenies and showed the heterozygotic status of mulberry varieties.

Keywords : Morus spp , RAPD markers inheritance, molecular diversity.

The mulberry ( Morus spp .) is an economically important plant, as it is the only

food source of the domesticated Silkworm, Bombyx mori L. Mulberry is reported

to be distributed in 30 countries. According to Western historian, mulberry culture

spread to India from China through Kotar (Tibet) by about 140 B.C. Since then

the sericulture industry has widely grown and today it provides livelihood to

approximately seven million people in the country. The genus Morus belongs

to family Moraceae and it comprises of many species and varieties. There is

considerable amount of confusion in the taxonomy of the genus as adopted by

different taxonomist. The classification of mulberry more relined on the external