Genetic diversity analysis and RAPD marker

number of amplicons in x entry and ‘Ny’ is the total number of amplicons in y

entry. Genetic distance (1-s) was calculated and a dendrogram was constructed

based on the distance matrix data sets by applying unweighed pair group method

and arithmetic average (UPGMA) using PHYLIP (Phylogeny inference package)

version 3.6 (alpha 2). RAPD amplicon markers were identified by the source of

primers (OP=Operon) kit letter, the primer no. and its approximate size in base

pairs. Goodness of fit to the 3:1 and 1:1 pattern of segregation of RAPD markers,

as predicted for the Mendelian characters in a monohybrid cross and test cross

respectively, was determined by the Chi-square test.

Results and Discussion

Mysore Local (♀) and V-1 (♂) represented a pair of contrasting genotypes for

different morpho-agronomical characters ideally suited for genetically analysis

(Table 2). In general, Mysore Local is a low yielder but hardy and nutritionally

less superior. Whereas, V-1 is a recently evolved variety with high leaf production,

table 2: morpho-agronomical characters of mysore Local and v-1

Name of the Parameters

mysore Local

v-1

Color of the matured shoot

Brown

Grey

Phyllotaxy

1/3

2/5

Lentical density/sq.cm

11.0

13.0

Lobation type

Medium Lobed

Unlobed

Lobation number

0-4

0

Leaf Nature

Heterophyllous

Homophyllous

Leaf texture

Chartaceous

Coraceous

Leaf shape

Ovate

Ovate-Lanceolate

Easiness for harvesting

Hard to harvest

Easy to harvest

Leaf length(cm)

14.35

25.40

Leaf width(cm)

11.64

17.50

Petiole length (cm)

4.03

5.5

Petiole width(cm)

0.28

0.4

Length of the longest shoot(cm)

125.55

155.10

Total shoot length(cm)

1127.50

1605.00

Girth of the stem(cm)

6.86

10.55

Internodal distance(cm)

3.59

5.86

Yield/Plant(g)

400.77

695.36

Moisture% of leaf

68.14

74.66

Resistance of leaf spot

Moderately resistant

Resistant

Resistance to Powdery Mildew

Highly suspecptible

Moderately resistant

Int. J. Bioinformatics and Biological Sci.: v.2 n.1&2, p. 23-39. March & June 2014

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