Prakash et al.
markers namely OPA-02 2900 , OPA-03 900 and OPA-03 800 (Table 4). Besides, three
other RAPD markers OPS-04 1450 , OPA-04 1000 and OPA-07 1000 showed Mendelian
segregation for 1:1 ratio with non-significant Chi-square value of probability.
The banding intensity in the RAPD profiles varied from intense to normal and
feeble. However, no attempt was made to assign the genotype of the parents and
F 1 progenies because minor deviation in the RAPD reaction and electrophoresis
can adversely effect the interpretation. The genetic distance in comparision with
female parent (Mysore Local) was maximum in case of progenies P 41 (0.2) and
minimum (0.017) was in case of P 26 (Table 5). Similarly genetic distance in
comparison with male parent (V-1) was maximum in case of progenies P 12 and
P 19 (0.177) and minimum was in case of P 36 (0.040). Among the progenies the
maximum genetic distance was recorded between the progenies P 4 and P 38 (0.276)
and no genetic distance as per RAPD marker data was observed in between two
pairs of progenies namely P 4 and P 38 .
table 4: f 1 Segregation pattern of RaPD markers in mysore Local X v-1 cross
f1 Segregation
RaPD
marker Size
X2
X2
total
Primer
(in bp)
Presence
absence
(3:1)
(1:1)
(1)
(0)
3100
5
37
42
89.17*
24.38*
OPA – 01
1800
9
33
42
64.29*
13.71*
1050
14
28
42
38.89*
4.67*
OPA – 02
2900
29
13
42
0.79NS
10.76*
900
29
13
42
0.79NS
10.76*
800
33
9
42
0.28NS
24.47*
OPA – 03
700
17
25
42
26.7*
26.00*
600
17
25
42
26.7*
26.00*
1450
18
24
42
23.14*
0.86NS
OPA – 04
1300
11
31
42
53.36*
9.52*
1000
19
23
42
19.84*
0.38NS
OPA – 05
1000
16
26
42
30.51*
2.38*
The genetic distance between parents based on the RAPD data set is calculated
0.186, which is low in comparison with morpho-agronomical differentiation. This
may be attributed to the screening of a small fraction of genome by RAPD primer,
which may not reflect the genetic divergence between the varieties accurately. But
32
Int. J. Bioinformatics and Biological Sci.: v.2 n.1&2, p. 23-39. March & June 2014