Genetic diversity analysis and RAPD marker

Lack of genetic information and availability of markers have severely restricted

the mulberry-breeding programme. The study showed RAPD markers can be a

valuable tool for mulberry breeding programme. The study established an accurate

and reproducible profiling pattern for mulberry for plant variety identification

and protection of Plant Breeders Rights. The present study has made no attempt

to link the RAPD markers with the traits for breeders’ interest. The study needs

to be further strengthened by segregation analysis of RAPD markers in large

number of progenies and screening of many more primers for the development of

molecular linkage map. The progenies also need to be characterized for important

morpho-agronomical traits accurately. The establishment of correlation between

the amplification products and the morpho-agronomical traits by bulk segregation

analysis will help the breeders in identification of markers for the traits of their

interest. However, the present work has laid a foundation for future research in this

direction. The polymorphic RAPD markers in parents segregate as independent

alleles in the F ₁ progenies and also throwing some light on the heterozygotic

status of mulberry varieties. The findings suggested that RAPD can be used as a

technique for marker assisted selection (MAS) in mulberry breeding.

acknowledgement

The financial support provided under project by Central Sericultural Research and

Training Institute (CSR and TI), Govt. of India, Mysore is highly acknowledged to

carry out the research programme.

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