Optimization of fermentation parameters
table 1: comparative performance of the two strains with various parameters.
Maximum growth in cell
Max. ethanol produced in % g/v
number× 10 8
(Time in
Parameter
(Time in hours)
hours)
R3DPMP
R3DSC5
R3DPMP
R3DSC5
5
9.1±0.4 (12) 10.9±0.8 (36) 6.9±0.2 (18)
9.8±0.2 (48)
Inoculum
6
9.4±0.6 (09) 10.8±0.2 (36) 7.4±0.8 (12)
10.8±0.3 (48)
density in cell
7
9.5±0.3 (09) 10.9±0.7 (24) 8.4±0.2 (12)
12.9±0.14 (36)
number× 10 8
8
11.2±1 (18) 11.8±0.3 (24) 8.3±0.35 (12) 13.3±0.14 (36)
9
11±0.4 (12) 11.4±0.5 (24) 8.4±0.2 (12)
13.1±0.14 (36)
7.9±0.8
5
8.8±2 (0)
2.2±0.9 (09)
2.3±0.8 (24)
(0 and 09)
9 . 2 ± 0 . 0 8
10
9.9±1 (12)
4.8±0.9 (48)
5.0±0.6 (36)
% of glucose
(09)
in g/v
15 9.9±0.7 (12) 10±.9 (12)
6.2±0.5 (48)
7.3±0.6 (36)
Concentration
1 1 . 2 ± 0 . 1
20
9.9±0.1 (12)
8.3±0.3 (12)
9.5±0.8 (36)
(18)
25 9.3±0.5 (09) 11.8±0.3 (24) 10.1±0.4 (24) 12.5±0.2 (36)
30 -
11±0.4 (36)
-
13±0.5 (36)
30 11.3±2 (24) 11.4±0.1 (24) 8.3±0.34 (12) 12.2±0.8 (36)
35 10.3±1 (12) 11.4±0.5 (24) 8.8±0.17 (24) 11.8±0.6 (24)
Temperature
in °C
8.8±0.5 (0 and
40 8.5±0.7 (09)
8.7±0.28 (18) 8.7±0.5 (24)
09)
45 7.5±0.09 (0) 9.3±0.3 (0)
-
-
3
10±0.65 (09) 11.3±2 (48)
8.0±1.0 (15)
10.5±0.1 (48)
1 0 . 8 ± 0 . 5
5
11.5±1.4 (24) 8.7±0.6 (12)
12.1±0.4 (36)
Initial pH of
(06)
the medium
7
11±0.61 (12) 11.6±1.1 (24) 8.3±0.3 (12)
11.4±0.1 (36)
1 0 . 4 ± 0 . 4 1
9
11.4±0.6 (48) 8.7±0.56 (15) 11.6±0.1 (48)
(12)
Int. J. Bioinformatics and Biological Sci.: v.2 n.1&2, p. 71-83. March & June 2014
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