Kapoor et al.
  • Lipemia (milky appearance of serum sample was looked for).
  • All the samples along with the requisition forms were analyzed. Sample rejection
    data with the pre-analytical variable responsible was noted down in a log book.
    The data was collected and summarized on monthly basis. 46,404 samples were
    analysed after which the study was closed.
    Results
    46,404 samples (24,404 OPD and 22,000 IPD) were analysed, it was seen that
    1077 samples (2.32%) were rejected due to some unfavourable pre analytical
    variable. However, repeat samples were demanded to complete reporting for these
    samples.
    Out of total 1077 samples being rejected, the cause of abandoning tests in 323
    samples was hemolysis, followed by clotted blood as being the second most
    frequent cause of rejection of samples as seen in 194 samples. Blood collection
    in wrong tube and due to addition of wrong preservative was seen as the cause of
    rejection in 247 samples. Due to insufficient sample volume total of 87 samples
    were redemanded for investigations to be performed. Inappropriate timing of
    collection of samples resulted in the rejection of 97 samples. Mislabelling or
    misidentification was seen as a preanalytical error in 76 samples. Lipemia was
    considered as the preanalytical variable responsible for rejection of 53 samples
    (Table 1 and Figure 1).
    Table 1. Frequency and nature of occurrence of preanalytical errors in 1077 rejected
    samples
    Pre-analytical Variable
    No. of Rejected Samples
    % of Rejected Samples
    Hemolysis
    323
    29.99
    Clotted Blood
    194
    18.01
    Wrong Tube
    129
    11.98
    Wrong Preservative
    118
    10.96
    Wrong Volume
    87
    8.08
    Mislabelled Samples
    76
    7.06
    Wrong Timing
    97
    9 .0
    Lipemia
    53
    4.92
    130
    International Journal of Bioinformatics and Biological Sciences : v.2 n.3&4. Dec. 2014