Kapoor et al.
Lipemia (milky appearance of serum sample was looked for).
All the samples along with the requisition forms were analyzed. Sample rejection
data with the pre-analytical variable responsible was noted down in a log book.
The data was collected and summarized on monthly basis. 46,404 samples were
analysed after which the study was closed.
Results
46,404 samples (24,404 OPD and 22,000 IPD) were analysed, it was seen that
1077 samples (2.32%) were rejected due to some unfavourable pre analytical
variable. However, repeat samples were demanded to complete reporting for these
samples.
Out of total 1077 samples being rejected, the cause of abandoning tests in 323
samples was hemolysis, followed by clotted blood as being the second most
frequent cause of rejection of samples as seen in 194 samples. Blood collection
in wrong tube and due to addition of wrong preservative was seen as the cause of
rejection in 247 samples. Due to insufficient sample volume total of 87 samples
were redemanded for investigations to be performed. Inappropriate timing of
collection of samples resulted in the rejection of 97 samples. Mislabelling or
misidentification was seen as a preanalytical error in 76 samples. Lipemia was
considered as the preanalytical variable responsible for rejection of 53 samples
(Table 1 and Figure 1).
Table 1. Frequency and nature of occurrence of preanalytical errors in 1077 rejected
samples
Pre-analytical Variable
No. of Rejected Samples
% of Rejected Samples
Hemolysis
323
29.99
Clotted Blood
194
18.01
Wrong Tube
129
11.98
Wrong Preservative
118
10.96
Wrong Volume
87
8.08
Mislabelled Samples
76
7.06
Wrong Timing
97
9 .0
Lipemia
53
4.92
130
International Journal of Bioinformatics and Biological Sciences : v.2 n.3&4. Dec. 2014