Isolation, Purification and Characterization of Oil Degrading Bacteria

Measurement of oil degradation

The slant in which oil was degraded, were separated. Using the pipette, all the oil

was sucked out from each slant. The amount of oil sucked from each slant was

measured and recorded.

Results and Discussion

When the petriplates were inoculated with serially diluted sample and left

for incubation, colonies of bacteria developed. From it different cultures were

separated and grown in different agar slants. From each sample two cultures were

prepared, such that there was a total of six slants. In these six slants, after 24 hours,

growth was observed. Figure 1 and figure 2 represents the two cultures obtained

from sample 1.

Figure 1

Figure 2

Figure 3 and figure 4 represents the initial level of oil in the two cultures from

sample 1.

International Journal of Bioinformatics and Biological Sciences : v.2 n.3&4. Dec. 2014

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