Development of a solid-state fermentation process for production of bacterial

10.

Wheat bran

102.33

119.48

150.54

154.17

11.

Potato peel

116.14

128.36

142.78

119.82

12.

Sweet potato peel

127.63

140.33

149.77

138.16

13.

Banana peel

115.39

147.28

152.47

133.26

14.

Sugarcane baggase

109.42

135.75

148.73

142.38

15.

Soybean husk

108.23

113.84

125.94

107.96

Substrate: F(cal) = 31.40 > F (tab) = 1.93 (S) at 5%

Extraction medium : F(cal) = 6.92 > F (tab) = 2.82 (S) at 5%

Figure 1: Production variation of α- amylase by Bacillus licheniformis from different

agrobased substrates under SSF

In industries there is an essential need for an economical and simple indigenous

system for producing high titers of alpha amylases. One alternative low cost and

feasible production method is the use of SSF. However, there are several factors,

which affect SSF processes. Among these, selection of a suitable strain, substrate

and selection of process parameters are crucial (Pandey et al., 2000). In the present

investigation, SSF was applied for the production of alpha amylase by using

Bacillus licheniformis . This study revealed high levels of α-amylase production

with Bacillus licheniformis on sugarcane baggase and wheat bran as the solid

substrates. In the present investigation wheat bran was found to be best for the

production of α- amylase. The productivity levels of α- amylase from wheat bran

were found to be high in other published reports of Babu and Satyanarayana

(1995); Mamo and Gressesse (1999); Ramdas et al. (1996); Ramesh and Lonsane

(1987).The suitability of a particular substrate in a SSF process for the production

of α-amylase appears to be governed by the physicochemical requirements of

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