Development of a solid-state fermentation process for production of bacterial

Table 2. Production of α- amylase under different Inoculum sizes of Bacillus licheniformis

S.No

Inoculum size (106CFU/ml)

Enzyme activity(IU)

1.

0.5

78.58

2.

1

104.46

3.

2

153.95

4.

4

169.38

5.

6

178.84

6.

8

100.35

r =0.306, tcal =0.64<ttab =2.77 at 5% (NS), Y = 4.29 X + 115.51

Figure 2. Alpha amylase production by Bacillus licheniformis under different

inoculums sizes in SSF

3.2.2 Effect of different moisture content of SSF medium on alpha amylase

production by Bacillus licheniformis

Moisture is one of the most important parameters in SSF that influence the

organism and thereby enzyme production. Low and high moisture levels of the

substrate affect the growth of the microorganism resulting in varying enzyme

production. The present study conducted solid state fermentation of wheat bran

by using Bacillus licheniformis under different moisture concentration. The

maximum α-amylase production (171.89 IU) was obtained when the initial

substrate moisture was 80%. A gradual increase in the production of α-amylase

was observed with increase in moisture content from 55-80%. Further increase in

moisture content of 5% resulted in a sharp decline of amylase enzyme production

(168.64 IU) (Table 3 and Figure 3).

International Journal of Bioinformatics and Biological Sciences : v.2 n.3&4. Dec. 2014

209