Development of a solid-state fermentation process for production of bacterial

Rameshkumar and Sivasudha (2011) also reported maximum yield of α- amylase

from rice bran at 80% moisture content by using Bacillus subtilis and obtained

decreased level of yield at further increase in moisture content. El-Shishtawy et

al. (2014) also reported the maximum α- amylase production from wheat bran

at moisture content of 80%. Nandakumar et al. (1996) obtained optimum α-

amylase production from wheat bran at 65% moisture content by using Bacillus

coagulans. Rai and Solanki (2014) reported highest amylase enzyme yield at 80%

while Tabassum et al. (2014) reported maximum production of α- amylase at

85% moisture content by using Bacillus licheniformis. Kalairasi and Parvatham

(2013) obtained the maximum enzyme production with moisture in the ratio of

1:2. Nagrajan et al. (2010) reported maximum amylase enzyme yield from wheat

bran at 88% moisture level by using Bacillus subtilis. Sameh et al. (2013) reported

maximum enzyme production from crude millet at 86% moisture content. Noreen

et al. (2002) reported highest α- amylase yield from banana peel at 84% moisture

content while Ashraf et al. (2005) obtained best results from wheat bran at the

moiture ratio 1:1 by using Bacillus licheniformis.

Effect of pH of SSF medium on alpha amylase production by Bacillus licheniformis

The initial pH of fermentation medium has a significant effect on bacterial growth

and enzyme production. The present study conducted the effect of pH on alpha

amylase enzyme production from wheat bran by Bacillus licheniformis. The

production medium was adjusted with different pH ranging from 4 to 10. From the

observation obtained in the present examination, maximum α-amylase production

(170.34 IU) was recorded at pH of 6.0. There was a 10.5% (152.46 IU) and 33.8%

(112.75 IU) decrease in activities at pH 7.0 and pH 8.0, respectively (Table 4 and

Fig 4).

Table 4. Production of alpha amylase by Bacillus licheniformis under different pH of SSF

medium

S.No

pH

Enzyme activity(IU)

1.

4

129.44

2.

5

142.85

3.

6

170.34

4.

7

152.46

5.

8

112.75

6.

9

81.14

7.

10

49.33

r =-0.767, tcal =-2.67 >ttab =2.57 at 5% (S), Y = 225.09 - 15.04 X

International Journal of Bioinformatics and Biological Sciences : v.2 n.3&4. Dec. 2014

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