Development of a solid-state fermentation process for production of bacterial

Effect of Incubation temperature on alpha amylase production by Bacillus

licheniformis

Normally the incubation temperature for the alpha amylase production is much

related to the growth of the microorganism. Therefore the optimum incubation

temperature for the fermentation depends on whether the microorganism is

mesophilic or thermophilic. The bacterial alpha amylase is produced at much

wider range of temperature. In the present study alpha amylase produced by

Bacillus licheniformis showed considerable enzyme activities in the range of 35°C

- 55°C. The enzyme activity increased upto 40°C (168.78 IU) and then the enzyme

activity dropped tremendously at 50°C and 55°C. The optimal temperature for

maximum amylase production (168.78 IU) was found to be 40°C (Table 5; Fig 5).

Table 5. Alpha amylase production by Bacillus licheniformis under different incubation

temperature of SSF medium

S.No

Incubation Temperature(oC)

Enzyme activity(IU)

1.

35

149.62

2.

40

168.78

3.

45

132.45

4.

50

99.32

5.

55

90.56

r =-0.89, tcal =-3.5>ttab =3.18 at 5% (S), Y = 296.96 - 3.75 X

Figure 5: Variation of alpha amylase production by Bacillus licheniformis under

different Incubation temperature of SSF

Similar results have been reported by Serin et al. (2012) for the production

of alpha amylase when rice bran was used with B. circulans at 37°C. Raj and

Hemashenpagam (2012) obtained maximum production of amylase at 55°C by

using Bacillus sp. It has also been reported that the metabolic heat generated

during microbial cultivation in SSF exerts harmful effects on the microbial

International Journal of Bioinformatics and Biological Sciences : v.2 n.3&4. Dec. 2014

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