Development of a solid-state fermentation process for production of bacterial

enzyme production behavior. Some carbon source supports good growth with low

enzyme production while other support good growth as well as enzyme secretion.

The present investigation was conducted to optimize fermentation medium with

carbon sources for industrial enzyme production. The experiment was conducted

on the production medium containing wheat bran and supplementation with

different carbon sources namely glucose, lactose, maltose, soluble starch at 1%

(w/v) concentration. The production medium was then fermented using Bacillus

licheniformis for the production of α- amylase. In the present study there was a

significant increase of amylase enzyme yield in media supplemented with maltose

(178.46 IU) whereas the production of α- amylase by B. licheniformis was

suppressed when bacterium was grown in the production medium with glucose

(142.44 IU) and lactose (148.68 IU) (Table 7;Fig 7).

Table 7. Production of alpha amylase by Bacillus licheniformis with additional carbon

sources supplemented to SSF

S.No

Carbon source

Enzyme activity(IU)

1.

Control

156.18

2.

Glucose (1%)

142.44

3.

Lactose (1%)

148.68

4.

Maltose (1%)

178.46

5.

Soluble starch (1%)

166.26

Fcal =606.48 > Ftab =11.25 at 1% (S)

Figure 7. Variation in α- amylase production by Bacillus licheniformis with additional

carbon sources supplementation

Similar result was also recorded by Babu and Satyanarayana (1995) where glucose

did not enhance α- amylase production by Bacillus coagulans under solid state

fermentation using wheat bran. Lactose exhibited repressive effect on enzyme

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