Development of a solid-state fermentation process for production of bacterial

Effect of additional Organic Nitrogen sources for the alpha amylase production

by Bacillus licheniformis

Added nitrogen sources have been reported to have an inducing effect on the

production of various enzymes in SSF system.Addition of various organic nitrogen

sources namely peptone, tryptone, yeast extract, soy peptone to the medium was

investigated in the present study. In comparison with control there was significant

increase in enzyme yield in case of the supplementation of soy peptone. Among

the various organic nitrogen sources tested soy peptone at 1% showed maximum

alpha amylase yield (171.68 IU) followed by yeast extract (156.81 IU).Of the

nitrogen sources tested, soy peptone was the best inducer for alpha amylase

production while tryptone was a poor inducer. Peptone suppressed the amylase

activity (147.74 IU) (Table 8; Fig 8).

Table 8. Production of alpha amylase by Bacillus licheniformis due to additional Organic

Nitrogen sources supplementation

S.No

Nitrogen source

Enzyme activity(IU)

1.

Control

152.98

2.

Peptone (1%)

147.74

3.

Soy Peptone (1%)

171.68

4.

Yeast extract (1%)

156.81

5.

Tryptone (1%)

133.34

Fcal =592.24 < Ftab =11.25 at 1%, (S)

Figure 8. Variation in alpha amylase production by Bacillus licheniformis due to

additional Organic Nitrogen sources supplementation

The influence of yeast extract on α- amylase production might be due to the

presence of vitamin B- group, free amino acids and carbohydrates. Kalairasi and

International Journal of Bioinformatics and Biological Sciences : v.2 n.3&4. Dec. 2014

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