Development of a solid-state fermentation process for production of bacterial

supplementation into the medium. Ammonium sulphate, sodium nitrate and

ammonium nitrate were found to be inhibitory in amylase enzyme production

(Table 9; Figure 9).

Figure 9. Variation in alpha amylase production by Bacillus licheniformis due to

additional Inorganic Nitrogen sources supplementation

Addition of sodium nitrate to the wheat bran showing negative influence on the

production of α- amylase was also reported by Hassan and Karim (2012). The

repressing production of α- amylase is because of the nitrate (NO3-) compound

that is more difficult to be degraded into a simpler compound of nitrite (NO2-)

and subsequently into ammonium. As in ammonium chloride the nitrogen source

is readily utilized by Bacillus licheniformis. Thus the consumption of nitrogen

sources in ammonium chloride and sulphate is faster and more effective than that

in sodium nitrate.

Effect of different concentration of Phosphate on Alpha amylase production by

Bacillus licheniformis under SSF condition

Table 10: Production of alpha amylase by Bacillus licheniformis due to varying

concentration of Phosphate supplementation

S.No

Phosphate concentration

Enzyme activity(IU)

1.

Control

152.88

2.

0.01M

164.78

3.

0.02M

157.63

4.

0.03M

154.65

5.

0.04M

159.43

r =0.101, tcal =0.175<ttab =3.18 at 5% (NS), Y = 29.7 X + 157.28

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