Perspectives into probiotics- From phenotyping to pyrosequencing

helveticus MTCC5463andprobioticingredientslikeinulinandoligofructose

have been developed.

Carbonatedȱprobioticȱwheyȱdrinkȱwithȱartificialȱandȱnaturalȱcarbonationȱ

havebeendevelopedinadditiontotheabovementionedproductoverview.

It is further added that a Patent (Application No. 620/MUM/2011) has been

filedȱforȱinventionȱentitledȱ“processȱforȱmanufactureȱofȱherbalȱprobioticȱ

fermented milk product”.

Probiotics in a pouch

Selectedȱ strainsȱ fulfillingȱ mostȱ characteristicsȱ wereȱ preservedȱ andȱ usedȱ

inȱ differentȱ probioticȱ foodȱ preparations.ȱ Itȱ wasȱ thoughtȱ appropriateȱ toȱ

make them available as regular ready to use food supplements. Hence,

the search for suitable carrier media started. As a partial replacement

ofȱmilkȱsolids,ȱgram,ȱmoongȱandȱwheatȱfloursȱwereȱtried.ȱTheȱculturesȱ

wereȱ blendedȱ inȱ differentȱ mediaȱ andȱ driedȱ underȱ vacuumȱ andȱ sprayȱ

dried for direct commercial practice. However, maintaining viability

wasȱtheȱbottleneck.ȱInvestigationsȱintoȱreducingȱprocessingȱstressȱledȱtoȱ

the standardization of a spray drying process incorporating tomato and

banana in the fermented milk which gave highest possible survival of

about 14% in the dried preparation. Similar studies were conducted on

spray drying of standard strain of Lb. acidophilus in a mix containing wheat

malt, fermented milk, sugar and cocoa. The next venture was towards

development of milk-cereal blends. The cost of the product was reduced

by replacing a part of costly milk solids with cereal solids and secondly,

the nutritive value was enhanced due to the complimentary action of

milk and cereal nutrients. The modes of packaging the material was also

delved upon and spray dried acidophilus-malt preparation was packed

inȱ pouchesȱ ofȱ threeȱ differentȱ flexibleȱ packagingȱ materials.ȱAtmosphericȱ

storage upto two months with satisfactory viability and moisture control

could be guaranteed using polyethylene-aluminium foil laminate with a

logicalȱcostȱbenefitȱratio.

Our Probiotic in pharma dosage forms

The increasing application of starter cultures and probiotics demand them

toȱbeȱmadeȱavailableȱtoȱtheȱconsumersȱinȱdifferentȱdosageȱforms,ȱwhichȱ

can provide ease of handling, ease of addition to food, precise dosage and

functionalityȱforȱspecificȱapplicationȱandȱlongȱtermȱpreservation.ȱInȱtheȱ

current study freeze dried cultures of Streptococcus thermophilus MTCC

5460, Lactobacillus helveticus MTCC 5463, Lactobacillus rhamnosus MTCC

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