Prajapati and Senan

5462 and Lactobacillus delbrueckii subsp. bulgaricus NCIM 2358 were used

for preparation of dosage forms viz., sachets, capsules and tablets for

use in the household and industrial level either as inocula for product

preparation or as food ingredient or dietary supplement.

The dosage forms at refrigerated storage exhibited good viability, activity

and physical characteristics for a period of six months. The viable counts

of individual cultures in sachets and capsules made of all three culture

combinations were found to be more than 9 log cfu/dosage form even after

six months of storage at refrigeration temperature. Tablets of all AIs also

showed a viable count of 8 log units after 6 months of storage indicating the

suitability of tablets for use as inocula for product preparation provided

that the tablets are manufactured under good hygienic conditions The

physicalȱparametersȱofȱtheȱdevelopedȱtabletsȱsatisfiedȱtheȱtechnologicalȱ

requirements needed for their easy adaptations to the industrial

manufacturing. For making dahi at household level, one unit of dosage

form, i.e., 1 sachet/1 capsule/1tablet of 300 mg as inocula per 100 ml of milk

andȱincubationȱatȱ37⁰Cȱforȱovernightȱ(12ȱtoȱ14ȱh)ȱisȱrecommended.

Taking the rubber to the road: Commercialization

A probiotic lassi containing our probiotic and dairy starter culture was

optimized for commercial production at Malabar Milk Union, Calicut

and the technology for the same has been transferred. The culture L.

helveticus MTCC 5463 has been licensed to Dr Baboo’s Food Science

and Biotechnology Pvt. Ltd, Thiruvanantapuram for commercial use.

Theȱ companyȱ willȱ useȱ andȱ marketȱ theȱ strainȱ inȱ differentȱ productsȱ andȱ

formats.ȱOneȱofȱtheȱfirstȱformulationȱisȱoatȱbasedȱprobioticȱlassi,ȱwhichȱ

isȱrichȱinȱβ-glucan,ȱwasȱdevelopedȱunderȱcollaborativeȱprojectȱwithȱLundȱ

University, Sweden. This product was launched on 6th December 2013

in an International conference by the State Agriculture Minister. We are

hopeful to provide health claims for this product in near future. In 2014

we were awarded Hari Ohm Ashram sponsored Prof. JP Trivedi award for

“DevelopmentȱofȱfirstȱIndianȱprobioticȱcultureȱtillȱcommercialȱapplication”ȱ

conferred by Gujarat Association of Agricultural Scientists (GAAS). Our

latestȱworkȱonȱmetagenomicsȱofȱhumanȱgutȱfloraȱasȱaffectedȱbyȱprobioticȱ

interventionȱ inȱ geriatricȱ populationȱ gotȱ specialȱ attentionȱ atȱ Americanȱ

Dairy Science Association, Kansas and Harvard Medical School recently.

This has improved visibility of Anand on the world map.

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