Journal of Animal Research: v.4 n.2, p. 201-209. Dec. 2014

DOI Number: 10.5958/2277-940X.2014.00006.0

Influence of Season on Biochemical Attributes of

Bhadawari Buffalo Bull Semen: Effect of Temperature

and Humidity

Vijay Pandey, Rajesh Nigam, Pawanjit Singh, Ambika Sharma 1 ,

Atul Saxena, Dilip Kumar Swain 2 , Lokesh Sharma 3 and Shilpi Dixit 4

Department of Biochemistry, College of Veterinary Science and Animal Husbandry,

DUVASU, Mathura, INDIA

1 Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science

and Animal Husbandry, DUVASU, Mathura, INDIA

2 Department of Veterinary Physiology, College of Veterinary Science and Animal

Husbandry, DUVASU, Mathura, INDIA

3 Diploma Courses, College of Veterinary Science and Animal Husbandry, DUVASU,

Mathura, INDIA

4 College of Biotechnology, DUVASU, Mathura, INDIA

*Corresponding Author: V Pandey; Email:drvijaypandey@gmail.com

Received: 06 August, 2014

Accepted: 15 October, 2014

ABSTRACT

The present study was undertaken to establish the seasonal variations in semen

biochemical indices in Bhadawari bull. Four fertile, healthy adult Bhadawari bulls

aged between 2 to 4 years were used as semen donors. The study was conducted

over a period of six months and divided into three seasons as winter season

(February- March), dry summer season (April-May) and wet summer season

(June-July). Sixteen semen samples were collected in each season (four ejaculates

from each bull) by using Artificial Vagina and seminal plasma were harvested

by centrifugation. The results of the study showed gradual and significant rise

in values of ALT, AST, ALP, cholesterol and triglycerides and decline in values

of amylase with increase in ambient temperature and THI. Amylase showed

negative correlation and other indices showed positive correlation with ambient

temperature and THI. The ambient temperature and THI showed positive

correlation with biochemical attributes except amylase while humidity showed

negative correlation. It could be concluded from the study that temperature and

THI of season significantly affects the semen biochemical attributes of Bhadawari

bulls.

Keywords: Biochemical, Season, Temperature, Humidity, Semen, Bull, Buffalo