Growth Dynamics of Salmonella

RESULTS AND DISCUSSION

Isolate S ₂ : S ₂ had bacterial count of 3.5 x10 2 CFU/ml on

zero hrs and attained maximum bacterial population of 9.2

Isolation of Salmonella from various sources

× 10 8 CFU/ml after 30 hrs. The lag phase and log phase

Isolation of Salmonella was carried out from raw meat of

lasted for 6 and 24 hrs respectively and the generation

poultry (n = 50), poultry cloacal swabs (n = 50), eggs (n =

time for S ₂ was 36 min .

200) and human diarrheic cases (n = 50).

Isolate S ₃ : The initial bacterial count of the isolate was 3.6

Isolation and identification of Salmonella isolates from

× 10 2 CFU/ml. Lag phase was of 6 hrs duration and log

raw meat of poultry, poultry cloacal swabs, eggs and

phase lasted for 24 hrs. Maximum bacterial population of

human diarrheic cases

9.1 × 10 8 CFU/ml was attained after 30 hrs. Generation

time for S ₃ was 36.5 min .

Of the 50 meat samples collected from freshly slaughtered

poultry, salmonellae were isolated from three samples, the

incidence being 6 per cent. Similarly, out of fifty poultry

Growth of isolates, obtained from poultry cloacal

cloacal swabs that were screened for Salmonella, only

swabs, at 30°C at pH 6.5

four samples were positive and the prevalence being 8 per

Isolate S The initial bacterial count of the isolate was

4 :

cent. Likewise, 1 and 3 isolates of Salmonella could be

2.6 x10 2 CFU/ml. Lag phase was of 6 hrs duration and log

recovered from 200 eggs and 50 human diarrheic cases,

phase lasted for 24 hrs. Maximum bacterial population of

respectively, thus showing the prevalence of 0.5 per cent

8.9 × 10 8 CFU/ml was attained after 30 hrs. Generation

in eggs and 6 per cent in human diarrheic cases. The results

time for S ₄ was 36 min.

are presented in Table 2.

Isolate S : It had bacterial count of 2.1 × 10 3 CFU/ml on

5

zero hrs and attained maximum population of 7.4 × 10 9

Table 2. Isolation of Salmonella from various sources

CFU/ml after 30 hrs. The lag phase and log phase lasted

for 6 and 24 hrs respectively and the generation time was

No. of

Per cent

Type of sample

No. of isolates

samples

isolation

36.5 min.

3

Isolate S ₆ : The bacterial count of the isolate was 3.1 × 10 3

Raw poultry meat

50

6

CFU/ml on zero hrs. Lag phase was of 6 hrs duration and

(S ₁ , S , S )

2

3

log phase lasted for 24 hrs. Maximum bacterial population

Poultry cloacal

4

50

swabs

of 8.3 × 10 9 CFU/ml was attained after 30 hrs and the

8

( S ₄ , S , S ,

5

6

generation time was 36 min.

S ₇ )

Eggs

1

Isolate S ₇ : S ₇ had bacterial count of 3.2 × 10 3 CFU/ml

200

0.5

on zero hrs and attained maximum bacterial population of

( S ₈ )

8.6x10 9 CFU/ml after 30 hrs. The lag phase and log phase

Human diarrheic

3

lasted for 6 and 24 hrs respectively. Generation time for S

50

7

cases

6

( S , S , S ,)

9

10

11

was 36 min.

Growth of the isolates at 30 o C at pH 6.5

Growth of isolates, obtained from eggs, at 30°C at pH

6.5

Growth of isolates, obtained from raw poultry meat, at

30°C at pH 6.5

Isolate S ₈ : The initial bacterial count of the isolate was

1.5 × 10 2 CFU/ml. Lag phase was of 6 hrs duration and

Isolate S The initial bacterial count of the isolate was 2.8

1 :

log phase lasted for 24 hrs. Maximum bacterial population

x10 2 CFU/ml. Lag phase recorded was of 6 hrs duration

of 7.8 × 10 8 CFU/ml was attained after 30 hrs. Generation

and log phase lasted for 15 hrs. Maximum bacterial

time for S ₈ was 36.5 min.

population of 9.4 x10 9 CFU/ml was attained after 21 hrs.

The generation time for S ₁ obtained was 36.5 min.

Journal of Animal Research: v.5 n.1. April 2015

87