Patel et al.
represent potential replacements for antibiotics in the
casei, Streptococcus thermophilus, Streptococcus faecium,
animal food industry because of their reported ability to
Bifidobacterium bifidum, Torulopsis spp . and Aspergillus
reduce enteric disease in poultry and potential food borne
oryzae.
pathogen contamination of poultry or poultry products
(Reid and Friendship, 2002; Patterson and Burkholder,
Table 1 : Proportion of feed ingredients and nutrient composition
2003). Numerous studies showed that addition of
(%) of basal diet
probiotics have positive effects on growth rate, feed
utilization, feed efficiency and mortality rate (Sen et al.,
Proportions (%)
2012; Manal, 2012). However, the efficacy of probiotics
Ingredients
Pre-starter Starter
Finisher
depends upon the selection of more efficient strains,
(0-10 d)
(11-21 d) (22-42 d)
gene manipulation, combination of several strains and
the combination of probiotics and synergistically acting
Maize
50.28
54.92
60.38
components. The use of multi-strain probiotics seems to
Soyabean meal
42.21
36.73
31.18
be the best way of potentiating the efficacy of probiotics
Vegetable oil
3.56
4.33
4.85
as it beneficially affects the host by improving the growth-
promoting bacteria with competitive antagonism of
Dicalcium phosphate
1.93
1.97
1.71
pathogenic bacteria in the gastrointestinal tract. Hence,
Common salt
0.35
0.35
0.35
keeping in view multi-strain probiotics was used to
evaluate the effect of probiotics supplementation on
Limestone
0.97
1.01
0.93
growth performance, feed consumption, feed conversion
Maduramycine
0.05
0.05
0.05
ratio, carcass characteristics, mortality and economics of
Lipocare1
0.10
0.10
0.10
feeding in broiler chicks.
L-Lysine
0.17
0.15
0.14
DL-Methionine
0.15
0.15
0.07
MATERIALS AND METHODS
Vitamin premix2
0.05
0.05
0.05
Location
Mineral premix3
0.20
0.20
0.20
The present experiment was carried out for six weeks from
Total
100.02
100.01
100.01
20 th November to 31 th December, 2012 at a private farm
Nutrient composition
located at Modasa, Sabarkantha district, Gujarat.
ME (kcal/kg)
2800
2950
3020
Crude Protein (%)
22.90
21.30
19.10
Experimental Design
Calcium (%)
0.97
0.92
0.86
A day old broiler chicks of strain ‘Cobb400’ (n=240)
were randomly divided into 3 dietary treatment groups of
Phosphorus (%)
0.45
0.45
0.40
similar mean weight comprising 80 birds each and each
group divided into 4 sub-groups of 20 birds each. The
1 Lecithin treated with co-enzyme
experimental design consisted of three dietary treatments
2 Provides per kg of diet: 12500 IU vitamin A; 2500 IU vitamin
as T : Basal diet without probiotics supplementation
1
D3; 12 mg vitamin E; 1.5 mg vitamin K; 1.5 mg vitamin B 1 ; 5
(Control), T : Basal diet + Probiotics supplementation
mg vitamin B 2 ; 2 mg vitamin B 6 , 15 mcg vitamin B 12 ; 15 mg
2
niacin, 10 mg pantothenic acid and 0.5 mg folic acid
@ 50g/ton of feed and T 3 : Basal diet + Probiotics
3 Provides per kg of diet: 50 mg iron, 10 mg copper, 50 mg zinc,
supplementation @ 100g/ton of feed for 6 weeks.
80 mg manganese1 mg iodine and 0.2 mg selenium
The multi-strain probiotics was (Protexin*, Novartis
India Ltd.) was supplemented which contained both
Housing and management
bacteria and yeast. Each gram of probiotics contains 2
Birds were reared in deep litter system of housing under
× 10 9 CFU of Lactobacillus plantarum, Lactobacillus
uniform and standard managemental conditions. Strict
bulgaricus, Lactobacillus acidophilus, Lactobacillus
156
Journal of Animal Research: v.5 n.1. April 2015