Polymorphism in Exon-40 of FASN Gene in Lesser known Buffalo breeds of India

FASN activity. T2264A was reported to have significant

given by Mr. Subhash (TO, NBAGR) and Mr. Joginder

effects on economic traits (Morris et al ., 2007; Schennink

Singh from NBAGR, Karnal.

et al ., 2009; Zhang et al ., 2008). Moreover, Abe et al .

(2009) reported that T1950A was completely linked with

Table 1. Genotype and allele frequencies in different breeds of

W1955R in Japanese Black cattle. In recent years, genetic

river buffalo

studies have focused on the manipulation of unsaturated

fatty acid composition of livestock products which have

Genotype

Breed / Population

Allele frequency

healthier effects on human metabolism (Taniguchi et al.,

frequencies

2004; Mele et al., 2007; Moioli et al., 2007; Kgwatalala et

Gojri buffalo

AA = 0.375

A = 0.67

al., 2009). The mammalian FASN gene is widely regulated

(n=40)

AG = 0.600

G = 0.33

at both the transcriptional and post-transcriptional levels.

One of the main transcription factors involved in this

GG = 0.025

regulation is SP1 (Schweizer et al ., 2002). In addition,

Chhattisgarhi

AA = 0.325

A = 0.60

it has also been described that FASN gene expression

buffalo

is regulated post transcriptionally by mediating mRNA

(n=40)

AG = 0.550

G = 0.40

stability (Semenkovich et al ., 1993). With regards to RNA

GG = 0.125

transcription via 5’-UTR-mediated regulation, Sp1 within

5’-UTRs has been shown to modulate the transcription

rate of genes (Muredda et al ., 2003; Lee et al ., 2005).

REFERENCES

Abe, T., J. Saburi, H. Hasebe, T. Nakagawa, T. Kawamura,

CONCLUSION

K. Saito, T. Nade, S. Misumi, T. Okumura, K. Kuchida, T.

Hayashi, S. Nakane, T. Mitsuhas, K. Nirasawa, Y. Sugimoto

It can be concluded that genetic variation is present

and E. Kobayashi. 2008. Bovine QTL analysis for growth,

in the coding region of FASN gene with adenine to

carcass, and meat quality traits in an F2 population from a

guanine transition in lesser known buffalo populations

cross between Japanese Black and Limousin. J. Anim. Sci.,

(Gojri and Chhattisgarhi buffalo) is being reported

86(11) : 2821-2832.

first time. Results suggest that exon 40 region of

Biochard, D., Grohs, C., Bourgeois, F., Cerqueira, F., Faugeras,

FASN gene in Gojri and Chhattisgarhi buffaloes is

R., Neau, A., Rupp, R., Amigues, Y., Boscher M.Y. and

Leveziel, H. 2003. Detection of genes influencing economic

highly polymorphic and sufficient genetic variability

traits in three French dairy cattle breeds. Genet. Selec. Evol.

is present in this fat related gene in contrast to

35(1) : 77-101.

other well-known candidate gene for fat percentage

Chakravarty, B., Gu, Z., Chirala, S.S., Wakil, S.J., Quiocho, F.A.

(DGAT) in buffaloes. This A to G transition can be

2004. Human fatty acid synthase: structure and substrate

used further as SNP markers, which could be helpful

selectivity of the thioesterase domain. Proc. Natl. Acad. Sci.

to breeders for future association studies, selecting

U. S. A., 101 : 15567–15572.

superior germplasm and conservation strategies. A

Chirala S.S., Chang H., Matzuk M., Abu-Elheiga L., Mao J.,

detailed analysis of other SNPs localized within this gene,

Mahon K., Finegold M. and Wakil S.J. 2003. Fatty acid

could possibly allow for indicating quantitative trait loci

synthesis is essential in embryonic development: fatty acid

for milk fat, which can be used in marker assisted selection

synthase null mutants and most of the heterozygotes die in

of river buffalo.

utero. Proc. Natl. Acad. Sci. U. S. A., 100 : 6358–63.

Falconer, D.S and Mackay, T.F.C. 1996. Introduction to

quantitative genetics. 4 th edn . Addison Wesley Longman

ACKNOWLEDGEMENTS

Ltd. Essex, England.

Authors thank the Director, NBAGR, Karnal (Haryana) for

Morris, C. A., N. G. Cullen, B. C. Glass, D. L. Hyndman, T. R.

providing the necessary facilities and ICAR for providing

Manley, S. M. Hickey, J. C. McEwan, W. S. Pitchford, C.

financial support. Thanks to Incharge BG lab, NBAGR for

D. Bottema and M. A. Lee. 2007. Fatty acid synthase effects

providing primers for the study. Authors thank the support

on bovine adipose fat and milk fat. Mamm. Genome 18(1) :

64-74.

Journal of Animal Research: v.5 n.2. June 2015

327